Multifocus structured illumination microscopy for fast volumetric super-resolution imaging

• We here report for the first time the synergistic implementation of structured illumination microscopy (SIM) and multifocus microscopy (MFM). This imaging modality is designed to alleviate the problem of insufficient volumetric acquisition speed in superresolution biological imaging. SIM is a wide-field super-resolution technique that allows imaging with visible light beyond the classical diffraction limit. Employing multifocus diffractive optics we obtain simultaneous wide-field 3D imaging capability in the SIM acquisition sequence, improving volumetric acquisition speed by an order of magnitude. Imaging performance is demonstrated on biological specimens.

Ämnesord

NATURVETENSKAP  -- Fysik -- Atom- och molekylfysik och optik (hsv//swe)

NATURAL SCIENCES  -- Physical Sciences -- Atom and Molecular Physics and Optics (hsv//eng)

TEKNIK OCH TEKNOLOGIER  -- Medicinteknik -- Medicinsk laboratorie- och mätteknik (hsv//swe)

ENGINEERING AND TECHNOLOGY  -- Medical Engineering -- Medical Laboratory and Measurements Technologies (hsv//eng)

Nyckelord

Fluorescence microscopy

Imaging systems

Microscopy

Superresolution

Three-dimensional microscopy

Diffraction

Fluorescence

Image reconstruction

Light

Mergers and acquisitions

Microscopic examination

Optical resolving power

Biological specimens

Imaging performance

Structured illumination microscopies (SIM)

Structured illumination microscopy

Super resolution

Super resolution imaging

Volumetric acquisitions

Magnetic force microscopy

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