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- Hellström, CeciliaKTH,Proteomik och nanobioteknologi,Science for Life Laboratory, SciLifeLab,Affinity Proteomics (author)
High-density serum/plasma reverse phase protein arrays
- Article/chapterEnglish2017
Publisher, publication year, extent ...
- 2017-07-04
- New York, NY :Humana Press,2017
- printrdacarrier
Numbers
- LIBRIS-ID:oai:DiVA.org:kth-216337
- https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-216337URI
- https://doi.org/10.1007/978-1-4939-7057-5_18DOI
Supplementary language notes
- Language:English
- Summary in:English
Part of subdatabase
- SwePubSwePub
Classification
Notes
- QC 20171020
- In-depth exploration and characterization of human serum and plasma proteomes is an attractive strategy for the identification of potential prognostic or diagnostic biomarkers. The possibility of analyzing larger numbers of samples in a high-throughput fashion has markedly increased with affinity-based microarrays, thus providing higher statistical power to these biomarker studies. Here, we describe a protocol for high-density serum and plasma reverse phase protein arrays (RPPAs). We demonstrate how a biobank of 12,392 samples was immobilized and analyzed on a single microarray slide, allowing high-quality profiling of abundant target proteins across all samples in one assay.
Subject headings and genre
- NATURVETENSKAP Kemi Analytisk kemi hsv//swe
- NATURAL SCIENCES Chemical Sciences Analytical Chemistry hsv//eng
- Affinity proteomics
- Fluorescent detection
- Noncontact inkjet printer
- Plasma
- Protein profiling
- Reverse phase protein array
- RPPA
- Serum
Added entries (persons, corporate bodies, meetings, titles ...)
- Dodig-Crnković, TeaKTH,Proteomik och nanobioteknologi,Science for Life Laboratory, SciLifeLab,Affinity Proteomics(Swepub:kth)u1wrth1x (author)
- Hong, Mun-GwanKTH,Science for Life Laboratory, SciLifeLab,Affinity Proteomics(Swepub:kth)u19gmsru (author)
- Schwenk, JochenKTH,Proteomik och nanobioteknologi,Science for Life Laboratory, SciLifeLab,Affinity Proteomics(Swepub:kth)u1h7wtme (author)
- Nilsson, PeterKTH,Proteomik och nanobioteknologi,Science for Life Laboratory, SciLifeLab,Affinity Proteomics(Swepub:kth)u1ws88sk (author)
- Sjöberg, RonaldKTH,Proteomik och nanobioteknologi,Science for Life Laboratory, SciLifeLab,Affinity Proteomics(Swepub:kth)u1j1wpi6 (author)
- KTHProteomik och nanobioteknologi (creator_code:org_t)
Related titles
- In:Serum/Plasma ProteomicsNew York, NY : Humana Press, s. 229-238
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