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Direct solid-phase ...
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Berg, Cecilia,1969-KTH,Proteomik och nanobioteknologi
(author)
Direct solid-phase sequence analysis of the human p53 gene by use of multiplex polymerase chain reaction and alpha-thiotriphosphate nucleotides.
- Article/chapterEnglish1995
Publisher, publication year, extent ...
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1995-10-01
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Oxford University Press (OUP),1995
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printrdacarrier
Numbers
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LIBRIS-ID:oai:DiVA.org:kth-219966
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https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-219966URI
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https://doi.org/10.1093/clinchem/41.10.1461DOI
Supplementary language notes
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Language:English
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Summary in:English
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Classification
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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QCR 20171219
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Among the candidate cancer-prognostic genes is the p53 tumor suppressor gene, which, when mutated, plays an important role in the development of many types of cancers. To facilitate robust large-scale DNA analysis of microdissected tumor biopsies, we describe a multiplex/nested PCR approach for a simultaneous outer amplification of exons 4-9 of the human p53 gene with parallel amplification of the HLA-DQB1 locus, involving a total of 14 primers. This approach reduces the required number of cells for analysis and avoids any variation in the amplifications of the individual p53 exons during the common outer amplification step. The HLA sequencing allows sample identification because the DQB1 locus is highly polymorphic and is thereby patient-specific. The p53 and HLA amplicons are analyzed by solid-phase sequencing in a semiautomated format. To improve the DNA sequence quality, we used 2'-deoxyribonucleoside 5'-O-1-thiotriphosphates in the sequencing reactions.
Subject headings and genre
Added entries (persons, corporate bodies, meetings, titles ...)
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Hedrum, A
(author)
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Holmberg, A
(author)
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Pontén, F
(author)
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Uhlén, M
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Lundeberg, J
(author)
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KTHProteomik och nanobioteknologi
(creator_code:org_t)
Related titles
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In:Clinical Chemistry: Oxford University Press (OUP)41:10, s. 1461-60009-91471530-8561
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