id:"swepub:oai:DiVA.org:kth-225308"
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- Säll, AnnaLund University,Lunds universitet,Institutionen för immunteknologi,Institutioner vid LTH,Lunds Tekniska Högskola,Department of Immunotechnology,Departments at LTH,Faculty of Engineering, LTH (author)
Advancing the immunoaffinity platform AFFIRM to targeted measurements of proteins in serum in the pg/ml range
- Article/chapterEnglish2018
Publisher, publication year, extent ...
- 2018-02-13
- PUBLIC LIBRARY SCIENCE,2018
- printrdacarrier
Numbers
- LIBRIS-ID:oai:DiVA.org:kth-225308
- https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-225308URI
- https://doi.org/10.1371/journal.pone.0189116DOI
- https://lup.lub.lu.se/record/e2e34d95-f44a-47ad-9ce8-69f4430cd75eURI
Supplementary language notes
- Language:English
- Summary in:English
Part of subdatabase
- SwePubSwePub
Classification
Notes
- QC 20180404
- There is a great need for targeted protein assays with the capacity of sensitive measurements in complex samples such as plasma or serum, not the least for clinical purposes. Proteomics keeps generating hundreds of biomarker candidates that need to be transferred towards true clinical application through targeted verification studies and towards clinically applicable analysis formats. The immunoaffinity assay AFFIRM (AFFInity sRM) combines the sensitivity of recombinant single chain antibodies (scFv) for targeted protein enrichment with a specific mass spectrometry readout through selected reaction monitoring (SRM) in an automated workflow. Here we demonstrate a 100 times improved detection capacity of the assay down to pg/ml range through the use of oriented antibody immobilization to magnetic beads. This was achieved using biotin-tagged scFv coupled to streptavidin coated magnetic beads, or utilizing the FLAG tag for coupling to anti-FLAG antibody coated magnetic beads. An improved multiplexing capacity with an 11-plex setup was also demonstrated compared to a previous 3-plex setup, which is of great importance for the analysis of panels of biomarker targets.
Subject headings and genre
- NATURVETENSKAP Kemi Analytisk kemi hsv//swe
- NATURAL SCIENCES Chemical Sciences Analytical Chemistry hsv//eng
- MEDICIN OCH HÄLSOVETENSKAP Medicinska och farmaceutiska grundvetenskaper Immunologi inom det medicinska området hsv//swe
- MEDICAL AND HEALTH SCIENCES Basic Medicine Immunology in the medical area hsv//eng
- TEKNIK OCH TEKNOLOGIER Medicinteknik Medicinsk laboratorie- och mätteknik hsv//swe
- ENGINEERING AND TECHNOLOGY Medical Engineering Medical Laboratory and Measurements Technologies hsv//eng
Added entries (persons, corporate bodies, meetings, titles ...)
- Corbee, DanielLund Univ, Dept Immunotechnol, Lund, Sweden.;Camurus, Lund, Sweden.,Lund University (author)
- Vikstrom, SaraLund Univ, Dept Immunotechnol, Lund, Sweden.;BioGaia AB, Lund, Sweden.,Lund University (author)
- Ottosson, FilipLund University,Lunds universitet,Institutionen för immunteknologi,Institutioner vid LTH,Lunds Tekniska Högskola,Department of Immunotechnology,Departments at LTH,Faculty of Engineering, LTH(Swepub:lu)immu-flo (author)
- Persson, HelenaKTH Royal Institute of Technology,KTH,Industriell bioteknologi,Science for Life Laboratory, SciLifeLab,Lund Univ, Dept Immunotechnol, Lund, Sweden.(Swepub:kth)u1l700sh (author)
- Waldemarson, SofiaLund University,Lunds universitet,Institutionen för immunteknologi,Institutioner vid LTH,Lunds Tekniska Högskola,Department of Immunotechnology,Departments at LTH,Faculty of Engineering, LTH(Swepub:lu)elma-bso (author)
- Institutionen för immunteknologiInstitutioner vid LTH (creator_code:org_t)
Related titles
- In:PLOS ONE: PUBLIC LIBRARY SCIENCE13:21932-6203
Internet link
- https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0189116&type=printable
- http://dx.doi.org/10.1371/journal.pone.0189116FULLTEXT
- https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-225308
- https://doi.org/10.1371/journal.pone.0189116
- https://lup.lub.lu.se/record/e2e34d95-f44a-47ad-9ce8-69f4430cd75e
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