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Expansion of Gammad...
Expansion of Gammadelta T Cells from Cord Blood : A Therapeutical Possibility
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- Berglund, Sofia (author)
- Karolinska Institutet
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- Gaballa, Ahmed (author)
- Karolinska Institutet
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- Sawaisorn, Piamsiri (author)
- Karolinska Inst, Dept Clin Sci Intervent & Technol, Stockholm, Sweden.;Mahidol Univ, Fac Med Technol, Ctr Res & Innovat, Bangkok, Thailand.
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- Sundberg, Berit (author)
- Karolinska Inst, Dept Clin Sci Intervent & Technol, Stockholm, Sweden.
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- Uhlin, Michael (author)
- Karolinska Institutet,KTH,Tillämpad fysik,Karolinska Inst, Dept Clin Sci Intervent & Technol, Stockholm, Sweden.; Karolinska Univ Hosp, Dept Immunol & Transfus Med, Stockholm, Sweden.
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Karolinska Institutet Karolinska Inst, Dept Clin Sci Intervent & Technol, Stockholm, Sweden;Mahidol Univ, Fac Med Technol, Ctr Res & Innovat, Bangkok, Thailand. (creator_code:org_t)
- HINDAWI LTD, 2018
- 2018
- English.
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In: STEM CELLS INTERNATIONAL. - : HINDAWI LTD. - 1687-966X .- 1687-9678.
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Abstract
Subject headings
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- Gammadelta (gamma delta) T cells are found in both blood and tissues and have antiviral and antitumor properties. The frequency of gamma delta T cells in umbilical cord blood (UCB) is low, and the majority express delta 1, in contrast to blood, whereas the main subset is delta 2 gamma 9 T cells. UCB gamma delta T cells are functionally immature, which together with their scarcity complicates the development of UCB gamma delta T cell therapies. We aimed to develop an effective expansion protocol for UCB gamma delta T cells based on zoledronate and IL-2. We found that culture with 5 mu M zoledronate and 200 IU IL-2/ml medium for 14 days promoted extensive proliferation. The majority of the cultured cells were gamma 9 delta 2 T cells. The fold expansion of this, originally infrequent, subset was impressive (median and maximum fold change 253 and 1085, resp.). After culture, the cells had a polyclonal gamma delta T cell repertoire and the main memory subset was central memory (CD45RO(+) CD27(+)). The cells produced cytokines such as IL-1B, IL-2, and IL-8 and displayed significant tumor-killing capacity. These results show that development of in vitro expanded UCB gamma delta T cell therapies is feasible. It could prove a valuable treatment modality for patients after umbilical cord blood transplantation.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
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