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A comprehensive str...
A comprehensive structural, biochemical and biological profiling of the human NUDIX hydrolase family
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- Carreras-Puigvert, Jordi (author)
- Karolinska Institutet,Karolinska Inst, Div Translat Med & Chem Biol, Dept Mol Biochem & Biophys, Sci Life Lab, S-17165 Stockholm, Sweden
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- Zitnik, M. (author)
- Univ Ljubljana, Fac Comp & Informat Sci, SI-1000 Ljubljana, Slovenia.; Stanford Univ, Dept Comp Sci, Palo Alto, CA 94305 USA
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- Jemth, A. -S (author)
- Karolinska Institutet,Karolinska Inst, Div Translat Med & Chem Biol, Dept Mol Biochem & Biophys, Sci Life Lab, S-17165 Stockholm, Sweden
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- Carter, Megan (author)
- Stockholms universitet,Institutionen för biokemi och biofysik,Stockholm Univ, Dept Biochem & Biophys, S-10691 Stockholm, Sweden
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- Unterlass, J. E. (author)
- Karolinska Institutet,Karolinska Inst, Div Translat Med & Chem Biol, Dept Mol Biochem & Biophys, Sci Life Lab, S-17165 Stockholm, Sweden
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- Hallström, Björn M. (author)
- KTH,Science for Life Laboratory, SciLifeLab,KTH Royal Inst Technol, Sci Life Lab, Cell Profiling Affin Prote, S-17165 Stockholm, Sweden
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- Loseva, O. (author)
- Karolinska Institutet,Karolinska Inst, Div Translat Med & Chem Biol, Dept Mol Biochem & Biophys, Sci Life Lab, S-17165 Stockholm, Sweden
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- Karem, Z. (author)
- Karolinska Inst, Div Translat Med & Chem Biol, Dept Mol Biochem & Biophys, Sci Life Lab, S-17165 Stockholm, Sweden
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- Calderón-Montanõ, J. M. (author)
- Karolinska Inst, Div Translat Med & Chem Biol, Dept Mol Biochem & Biophys, Sci Life Lab, S-17165 Stockholm, Sweden
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- Lindskog, Cecilia (author)
- Uppsala universitet,Institutionen för immunologi, genetik och patologi,Science for Life Laboratory, SciLifeLab
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- Edqvist, Per-Henrik D (author)
- Uppsala universitet,Science for Life Laboratory, SciLifeLab,Experimentell och klinisk onkologi
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- Matuszewski, Damian J. (author)
- Uppsala universitet,Avdelningen för visuell information och interaktion,Science for Life Laboratory, SciLifeLab
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- Ait Blal, Hammou (author)
- KTH,Science for Life Laboratory, SciLifeLab,KTH Royal Inst Technol, Sci Life Lab, Cell Profiling Affin Prote, S-17165 Stockholm, Sweden
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- Berntsson, Ronnie P. A. (author)
- Stockholms universitet,Institutionen för biokemi och biofysik,Stockholm Univ, Dept Biochem & Biophys, S-10691 Stockholm, Sweden
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- Häggblad, Maria (author)
- Stockholms universitet,Institutionen för biokemi och biofysik,Science for Life Laboratory (SciLifeLab),Stockholm Univ, Dept Biochem & Biophys, Sci Life Lab, Biochem & Cellular Screening Facil, S-17165 Stockholm, Sweden
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- Martens, Ulf (author)
- Stockholms universitet,Institutionen för biokemi och biofysik,Science for Life Laboratory (SciLifeLab),Stockholm Univ, Dept Biochem & Biophys, Sci Life Lab, Biochem & Cellular Screening Facil, S-17165 Stockholm, Sweden
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- Studham, M. (author)
- Stockholms universitet,Institutionen för biokemi och biofysik,Science for Life Laboratory (SciLifeLab),Stockholm Univ, Dept Biochem & Biophys, Stockholm Bioinformat Ctr, Sci Life Lab, Box 1031, S-17121 Solna, Sweden
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- Lundgren, Bo (author)
- Stockholms universitet,Institutionen för biokemi och biofysik,Science for Life Laboratory (SciLifeLab),Stockholm Univ, Dept Biochem & Biophys, Sci Life Lab, Biochem & Cellular Screening Facil, S-17165 Stockholm, Sweden
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- Wählby, Carolina, 1974- (author)
- Uppsala universitet,Avdelningen för visuell information och interaktion,Science for Life Laboratory, SciLifeLab,Bildanalys och människa-datorinteraktion
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- Sonnhammer, Erik L. L. (author)
- Stockholms universitet,Institutionen för biokemi och biofysik,Science for Life Laboratory (SciLifeLab),Stockholm Univ, Dept Biochem & Biophys, Stockholm Bioinformat Ctr, Sci Life Lab, Box 1031, S-17121 Solna, Sweden
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- Lundberg, Emma (author)
- KTH,Science for Life Laboratory, SciLifeLab,KTH Royal Inst Technol, Sci Life Lab, Cell Profiling Affin Prote, S-17165 Stockholm, Sweden
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- Stenmark, Pål (author)
- Stockholms universitet,Institutionen för biokemi och biofysik,Stockholm Univ, Dept Biochem & Biophys, S-10691 Stockholm, Sweden
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- Zupan, B. (author)
- Univ Ljubljana, Fac Comp & Informat Sci, SI-1000 Ljubljana, Slovenia.; Baylor Coll Med, Dept Mol & Human Genet, Houston, TX 77030 USA
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- Helleday, T. (author)
- Karolinska Institutet,Karolinska Inst, Div Translat Med & Chem Biol, Dept Mol Biochem & Biophys, Sci Life Lab, S-17165 Stockholm, Sweden
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(creator_code:org_t)
- 2017-11-16
- 2017
- English.
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In: Nature Communications. - : Nature Publishing Group. - 2041-1723. ; 8:1
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https://doi.org/10.1...
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https://doi.org/10.1...
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Abstract
Subject headings
Close
- The NUDIX enzymes are involved in cellular metabolism and homeostasis, as well as mRNA processing. Although highly conserved throughout all organisms, their biological roles and biochemical redundancies remain largely unclear. To address this, we globally resolve their individual properties and inter-relationships. We purify 18 of the human NUDIX proteins and screen 52 substrates, providing a substrate redundancy map. Using crystal structures, we generate sequence alignment analyses revealing four major structural classes. To a certain extent, their substrate preference redundancies correlate with structural classes, thus linking structure and activity relationships. To elucidate interdependence among the NUDIX hydrolases, we pairwise deplete them generating an epistatic interaction map, evaluate cell cycle perturbations upon knockdown in normal and cancer cells, and analyse their protein and mRNA expression in normal and cancer tissues. Using a novel FUSION algorithm, we integrate all data creating a comprehensive NUDIX enzyme profile map, which will prove fundamental to understanding their biological functionality.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Medicinsk bioteknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Medical Biotechnology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
- NATURVETENSKAP -- Data- och informationsvetenskap -- Bioinformatik (hsv//swe)
- NATURAL SCIENCES -- Computer and Information Sciences -- Bioinformatics (hsv//eng)
Keyword
- Nudix hydrolase
- NUDT1 protein
- NUDT10 protein
- NUDT11 protein
- NUDT12 protein
- NUDT13 protein
- NUDT14 protein
- NUDT16 protein
- NUDT17 protein
- NUDT19 protein
- NUDT20 protein
- NUDT21 protein
- NUDT22 protein
- NUDT4 protein
- NUDT5 protein
- NUDT6 protein
- NUDT7 protein
- NUDT8 protein
- unclassified drug
- algorithm
- biochemical composition
- cancer
- enzyme
- enzyme activity
- gene expression
- homeostasis
- metabolism
- protein
- A-549 cell line
- Article
- breast cancer
- cancer cell line
- cancer tissue
- cell cycle
- cell cycle regulation
- cell survival
- colorectal cancer
- controlled study
- DNA content
- enzyme structure
- functional genomics
- gene interaction
- gene silencing
- human
- human cell
- human tissue
- immunohistochemistry
- melanoma
- phylogenetic tree
- protein expression
- sequence alignment
- tissue microarray
- upregulation
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
To the university's database
- By the author/editor
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Carreras-Puigver ...
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Zitnik, M.
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Jemth, A. -S
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Carter, Megan
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Unterlass, J. E.
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Hallström, Björn ...
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show more...
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Loseva, O.
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Karem, Z.
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Calderón-Montanõ ...
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Lindskog, Cecili ...
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Edqvist, Per-Hen ...
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Matuszewski, Dam ...
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Ait Blal, Hammou
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Berntsson, Ronni ...
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Häggblad, Maria
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Martens, Ulf
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Studham, M.
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Lundgren, Bo
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Wählby, Carolina ...
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Sonnhammer, Erik ...
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Lundberg, Emma
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Stenmark, Pål
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Zupan, B.
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Helleday, T.
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show less...
- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Medical Biotechn ...
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and Medical Biotechn ...
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Biochemistry and ...
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Computer and Inf ...
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and Bioinformatics
- Articles in the publication
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Nature Communica ...
- By the university
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Royal Institute of Technology
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Stockholm University
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Karolinska Institutet
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Uppsala University