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Galacturonate Metab...
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Valk, Laura C.Delft Univ Technol, Dept Biotechnol, Delft, Netherlands.
(författare)
Galacturonate Metabolism in Anaerobic Chemostat Enrichment Cultures : Combined Fermentation and Acetogenesis by the Dominant sp nov "Candidatus Galacturonibacter soehngenii"
- Artikel/kapitelEngelska2018
Förlag, utgivningsår, omfång ...
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American Society for Microbiology,2018
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printrdacarrier
Nummerbeteckningar
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LIBRIS-ID:oai:DiVA.org:kth-235115
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https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-235115URI
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https://doi.org/10.1128/AEM.01370-18DOI
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Språk:engelska
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Sammanfattning på:engelska
Ingår i deldatabas
Klassifikation
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Ämneskategori:ref swepub-contenttype
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Ämneskategori:art swepub-publicationtype
Anmärkningar
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QC 20180919
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Agricultural residues such as sugar beet pulp and citrus peel are rich in pectin, which contains galacturonic acid as a main monomer. Pectin-rich residues are underexploited as feedstocks for production of bulk chemicals or biofuels. The anaerobic, fermentative conversion of D-galacturonate in anaerobic chemostat enrichment cultures provides valuable information toward valorization of these pectin-rich feedstocks. Replicate anaerobic chemostat enrichments, with D-galacturonate as the sole limiting carbon source and inoculum from cow rumen content and rotting orange peels, yielded stable microbial communities, which were dominated by a novel Lachnospiraceae species, for which the name "Candidatus Galacturonibacter soehngenii" was proposed. Acetate was the dominant catabolic product, with formate and H-2 as coproducts. The observed molar ratio of acetate and the combined amounts of H-2 and formate deviated significantly from 1, which suggested that some of the hydrogen and CO2 formed during D-galacturonate fermentation was converted into acetate via the Wood-Ljungdahl acetogenesis pathway. Indeed, metagenomic analysis of the enrichment cultures indicated that the genome of "Candidatus G. soehngenii" encoded enzymes of the adapted Entner-Doudoroff pathway for D-galacturonate metabolism as well as enzymes of the Wood-Ljungdahl pathway. The simultaneous operation of these pathways may provide a selective advantage under D-galacturonate-limited conditions by enabling a higher specific ATP production rate and lower residual D-galacturonate concentration than would be possible with a strictly fermentative metabolism of this carbon and energy source. IMPORTANCE This study on D-galacturonate metabolism by open, mixed-culture enrichments under anaerobic, D-galacturonate-limited chemostat conditions shows a stable and efficient fermentation of D-galacturonate into acetate as the dominant organic fermentation product. This fermentation stoichiometry and population analyses provide a valuable baseline for interpretation of the conversion of pectin-rich agricultural feedstocks by mixed microbial cultures. Moreover, the results of this study provide a reference for studies on the microbial metabolism of D-galacturonate under different cultivation regimes.
Ämnesord och genrebeteckningar
Biuppslag (personer, institutioner, konferenser, titlar ...)
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Frank, JeroenRadboud Univ Nijmegen, Soehngen Inst Anaerob Microbiol, Nijmegen, Netherlands.
(författare)
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de la Torre-Cortes, PilarDelft Univ Technol, Dept Biotechnol, Delft, Netherlands.
(författare)
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van 't Hof, MaxDelft Univ Technol, Dept Biotechnol, Delft, Netherlands.
(författare)
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van Maris, Antonius J. A.,Professor,1976-KTH,Industriell bioteknologi,Delft Univ Technol, Dept Biotechnol, Delft, Netherlands.(Swepub:kth)u1n330r1
(författare)
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Pronk, Jack T.Delft Univ Technol, Dept Biotechnol, Delft, Netherlands.
(författare)
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van Loosdrecht, Mark C. M.Delft Univ Technol, Dept Biotechnol, Delft, Netherlands.
(författare)
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Delft Univ Technol, Dept Biotechnol, Delft, Netherlands.Radboud Univ Nijmegen, Soehngen Inst Anaerob Microbiol, Nijmegen, Netherlands.
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:Applied and Environmental Microbiology: American Society for Microbiology84:180099-22401098-5336
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