Study of interaction between red-tide toxin, domoic acid and double-stranded DNA by capillary zone electrophoresis

• The interactions between amnesic red-tide toxin, domoic acid (DA) and 14mer double-stranded DNA (dsDNA with three kinds of sequences) were studied by capillary zone electrophoresis (CZE). For the dsDNA with a sequence of 5'-CCCCCTATACCCGC-3', the amount of free dsDNA decreases with the increase of added DA; and the signal of DA-dsDNA complex was observed. Meanwhile, the other two dsDNAs, 5'-(C)12GC-3' and 5'-(AT)7-3', the existence of DA could not lead to the change of dsDNA signal and indicated that there is no interaction between DA and these two dsDNAs.

Nyckelord

DA

dsDNA

interaction

capillary zone electrophoresis

Publikations- och innehållstyp

ref (ämneskategori)

art (ämneskategori)