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Parallel single mol...
Parallel single molecule detection with a fully integrated single-photon 2X2 CMOS detector array
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Gosch, M. (författare)
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Serov, A. (författare)
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Anhut, T. (författare)
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Lasser, T. (författare)
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Rochas, A. (författare)
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Besse, P. A. (författare)
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- Popovic, R. S. (författare)
- Karolinska Institutet
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- Blom, Hans (författare)
- KTH,Fysik
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Rigler, R. (författare)
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(creator_code:org_t)
- SPIE-Intl Soc Optical Eng, 2004
- 2004
- Engelska.
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Ingår i: Journal of Biomedical Optics. - : SPIE-Intl Soc Optical Eng. - 1083-3668 .- 1560-2281. ; 9:5, s. 913-921
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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http://kipublication...
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Abstract
Ämnesord
Stäng
- We present parallel single molecule detection (SMD) and fluorescence correlation spectroscopy (FCS) experiments with a fully integrated complementary metal oxide semiconductor (CMOS) single-photon 2 X 2 detector array. Multifocal excitation is achieved with a diffractive optical element (DOE). Special emphasis is placed on parallelization of the total system. The performance of the novel single-photon CMOS detector is investigated and compared to a state-of-the-art single-photon detecting module [having an actively quenched avalanche photodiode (APD)] by measurements on free diffusing molecules at different concentrations. Despite the order of magnitude lower detection efficiency of the CMOS detector compared to the state-of-the-art single-photon detecting module, we achieve single molecule sensitivity and reliably determine molecule concentrations. In addition, the CMOS detector performance for the determination of the fraction of slowly diffusing molecules in a primer solution (two-component analysis) is demonstrated. The potential of this new technique for high-throughput confocal-detection-based systems is discussed.
Ämnesord
- TEKNIK OCH TEKNOLOGIER -- Elektroteknik och elektronik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Electrical Engineering, Electronic Engineering, Information Engineering (hsv//eng)
Nyckelord
- parallel single molecule detection
- single-photon detector
- complementary metal oxide semiconductor technology
- detector arrays
- parallel confocal spectroscopy
- fluorescence correlation spectroscopy
- fluorescence correlation spectroscopy
- fan-out element
- statistical accuracy
- flow
- fluctuations
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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