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An electronically t...
An electronically tunable ultrafast laser source applied to fluorescence imaging and fluorescence lifetime imaging microscopy
- Article/chapterEnglish2004
Publisher, publication year, extent ...
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2004-11-20
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IOP Publishing,2004
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printrdacarrier
Numbers
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LIBRIS-ID:oai:DiVA.org:kth-23971
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https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-23971URI
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https://doi.org/10.1088/0022-3727/37/23/011DOI
Supplementary language notes
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Language:English
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Summary in:English
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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QC 20100525
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Fluorescence imaging is used widely in microscopy and macroscopic imaging applications for fields ranging from biomedicine to materials science. A critical component for any fluorescence imaging system is the excitation source. Traditionally, wide-field systems use filtered thermal or arc-generated white light sources, while point scanning confocal microscope systems require spatially coherent (point-like) laser sources. Unfortunately, the limited range of visible wavelengths available from conventional laser sources constrains the design and usefulness of fluorescent probes in confocal microscopy. A 'hands-off' laser-like source, electronically tunable across the visible spectrum, would be invaluable for fluorescence imaging and provide new opportunities, e.g. automated excitation fingerprinting and in situ measurement of excitation cross-sections. Yet more information can be obtained using fluorescence lifetime imaging (FLIM), which requires that the light source be pulsed or rapidly modulated. We show how a white light continuum, generated by injecting femtosecond optical radiation into a micro-structured optical fibre, coupled with a simple prism-based tunable filter arrangement, can fulfil all these roles as a continuously electronically tunable (435-1150 nm) visible ultrafast light source in confocal, wide-field and FLIM systems.
Subject headings and genre
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photonic crystal fibers
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supercontinuum generation
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anomalous-dispersion
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continuum generation
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spatial-resolution
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raman-scattering
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optical-fiber
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multiphoton
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cell
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pulses
Added entries (persons, corporate bodies, meetings, titles ...)
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Lanigan, P. M. P.
(author)
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McGinty, J.
(author)
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Elson, D. S.
(author)
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Requejo-Isidro, J.
(author)
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Munro, I.
(author)
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Galletly, N.
(author)
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McCann, F.
(author)
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Treanor, B.
(author)
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Önfelt, Björn
(author)
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Davis, D. M.
(author)
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Neil, M. A. A.
(author)
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French, P. M. W.
(author)
Related titles
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In:Journal of Physics D: IOP Publishing37:23, s. 3296-33030022-37271361-6463
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Dunsby, C.
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Lanigan, P. M. P ...
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McGinty, J.
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Elson, D. S.
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Requejo-Isidro, ...
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Munro, I.
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Galletly, N.
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McCann, F.
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Treanor, B.
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Önfelt, Björn
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Davis, D. M.
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Neil, M. A. A.
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French, P. M. W.
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Journal of Physi ...
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Royal Institute of Technology