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Pilot-scale process...
Pilot-scale process for magnetic bead purification of antibodies directly from non-clarified CHO cell culture
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- Brechmann, Nils Arnold (author)
- KTH,Industriell bioteknologi,AdBIOPRO, VINNOVA Competence Centre for Advanced BioProduction by Continuous Processing, Stockholm, Sweden,Animal cell technology,AdBIOPRO, VINNOVA Competence Ctr Adv Bioprod Continuous Pro, Stockholm, Sweden;KTH Royal Inst Technol, Sch Engn Sci Chem Biotechnol & Hlth, Dept Ind Biotechnol, Cell Technol Grp CETEG, Stockholm, Sweden
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- Eriksson, Per-Olov (author)
- PE Bioproc Consulting AB, Strangnas, Sweden
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- Eriksson, Kristofer (author)
- AdBIOPRO, VINNOVA Competence Ctr Adv Bioprod Continuous Pro, Stockholm, Sweden;Lab On A Bead AB, Uppsala, Sweden
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- Oscarsson, Sven (author)
- Stockholms universitet,Institutionen för organisk kemi,Stockholm Univ, Dept Organ Chem, Stockholm, Sweden
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- Buijs, Jos (author)
- Uppsala universitet,Medicinsk strålningsvetenskap
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- Shokri, Atefeh (author)
- KTH,Industriell bioteknologi,AdBIOPRO, VINNOVA Competence Centre for Advanced BioProduction by Continuous Processing, Stockholm, Sweden,Animal cell technology,AdBIOPRO, VINNOVA Competence Ctr Adv Bioprod Continuous Pro, Stockholm, Sweden;KTH Royal Inst Technol, Sch Engn Sci Chem Biotechnol & Hlth, Dept Ind Biotechnol, Cell Technol Grp CETEG, Stockholm, Sweden
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- Hjälm, Göran (author)
- AdBIOPRO, VINNOVA Competence Ctr Adv Bioprod Continuous Pro, Stockholm, Sweden;Lab On A Bead AB, Uppsala, Sweden
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- Chotteau, Véronique, Docent, 1963- (author)
- KTH,Industriell bioteknologi,AdBIOPRO, VINNOVA Competence Centre for Advanced BioProduction by Continuous Processing, Stockholm, Sweden,Animal cell technology,AdBIOPRO, VINNOVA Competence Ctr Adv Bioprod Continuous Pro, Stockholm, Sweden;KTH Royal Inst Technol, Sch Engn Sci Chem Biotechnol & Hlth, Dept Ind Biotechnol, Cell Technol Grp CETEG, Stockholm, Sweden
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(creator_code:org_t)
- 2019-01-30
- 2019
- English.
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In: Biotechnology progress (Print). - : AIChE. - 8756-7938 .- 1520-6033.
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Abstract
Subject headings
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- High capacity magnetic protein A agarose beads, LOABeads PrtA, were used in the developmentof a new process for affinity purification of monoclonal antibodies (mAbs) from non-clarifiedCHO cell broth using a pilot-scale magnetic separator. The LOABeads had a maximum bindingcapacity of 65 mg/mL and an adsorption capacity of 25–42 mg IgG/mL bead in suspension for anIgG concentration of 1 to 8 g/L. Pilot-scale separation was initially tested in a mAb capture stepfrom 26 L clarified harvest. Small-scale experiments showed that similar mAb adsorptions wereobtained in cell broth containing 40 Å~ 106 cells/mL as in clarified supernatant. Two pilot-scalepurification runs were then performed on non-clarified cell broth from fed-batch runs of 16 L,where a rapid mAb adsorption ≥96.6% was observed after 1 h. This process using 1 L of magnetic beads had an overall mAb yield of 86% and 16 times concentration factor. After this single proteinA capture step, the mAb purity was similar to the one obtained by column chromatography, whilethe host cell protein content was very low, <10 ppm. Our results showed that this magnetic beadmAb purification process, using a dedicated pilot-scale separation device, was a highly efficientsingle step, which directly connected the culture to the downstream process without cell clarification.Purification of mAb directly from non-clarified cell broth without cell separation can providesignificant savings in terms of resources, operation time, and equipment, compared to legacy procedure of cell separation followed by column chromatography step.
Subject headings
- TEKNIK OCH TEKNOLOGIER -- Industriell bioteknik -- Bioprocessteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Industrial Biotechnology -- Bioprocess Technology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Medicinsk bioteknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Medical Biotechnology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Keyword
- magnetic beads
- purification
- monoclonal antibody
- pilot-scale
- downstream-bioprocess
- Bioteknologi
- Biotechnology
Publication and Content Type
- ref (subject category)
- art (subject category)
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