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Development of para...
Development of parallel reaction monitoring assays for cerebrospinal fluid proteins associated with Alzheimer's disease
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- Andersson, Annika (författare)
- KTH,Science for Life Laboratory, SciLifeLab,Proteinvetenskap
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- Remnestål, Julia (författare)
- KTH,Affinitets-proteomik,Science for Life Laboratory, SciLifeLab
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- Nellgård, Bengt, 1956 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper, Avdelningen för anestesiologi och intensivvård,Institute of Clinical Sciences, Department of Anesthesiology and Intensive care
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- Vunk, Helian (författare)
- KTH,Science for Life Laboratory, SciLifeLab,Proteinvetenskap
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- Kotol, David (författare)
- KTH,Science for Life Laboratory, SciLifeLab,Proteinvetenskap
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- Edfors, Fredrik (författare)
- KTH,Proteinvetenskap,Science for Life Laboratory, SciLifeLab
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- Uhlén, Mathias (författare)
- KTH,Science for Life Laboratory, SciLifeLab,Proteinvetenskap
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- Schwenk, Jochen M. (författare)
- KTH,Science for Life Laboratory, SciLifeLab,Proteinvetenskap
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- Ilag, Leopold L. (författare)
- Stockholms universitet,Institutionen för miljövetenskap och analytisk kemi
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- Zetterberg, Henrik, 1973 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi,Institute of Neuroscience and Physiology
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- Blennow, Kaj, 1958 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi,Institute of Neuroscience and Physiology
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- Månberg, Anna, 1985- (författare)
- KTH,Science for Life Laboratory, SciLifeLab,Proteinvetenskap
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- Nilsson, Peter (författare)
- KTH,Science for Life Laboratory, SciLifeLab,Proteinvetenskap
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- Fredolini, Claudia (författare)
- KTH,Science for Life Laboratory, SciLifeLab,Affinitets-proteomik
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(creator_code:org_t)
- Elsevier B.V. 2019
- 2019
- Engelska.
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Ingår i: Clinica Chimica Acta. - : Elsevier B.V.. - 0009-8981 .- 1873-3492. ; 494, s. 79-93
- Relaterad länk:
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https://doi.org/10.1...
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https://doi.org/10.1...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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https://urn.kb.se/re...
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https://gup.ub.gu.se...
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Abstract
Ämnesord
Stäng
- Detailed knowledge of protein changes in cerebrospinal fluid (CSF) across healthy and diseased individuals would provide a better understanding of the onset and progression of neurodegenerative disorders. In this study, we selected 20 brain-enriched proteins previously identified in CSF by antibody suspension bead arrays (SBA) to be potentially biomarkers for Alzheimer's disease (AD) and verified these using an orthogonal approach. We examined the same set of 94 CSF samples from patients affected by AD (including preclinical and prodromal), mild cognitive impairment (MCI), non-AD dementia and healthy individuals, which had previously been analyzed by SBA. Twenty-eight parallel reaction monitoring (PRM) assays were developed and 13 of them could be validated for protein quantification. Antibody profiles were verified by PRM. For seven proteins, the antibody profiles were highly correlated with the PRM results (r > 0.7) and GAP43, VCAM1 and PSAP were identified as potential markers of preclinical AD. In conclusion, we demonstrate the usefulness of targeted mass spectrometry as a tool for the orthogonal verification of antibody profiling data, suggesting that these complementary methods can be successfully applied for comprehensive exploration of CSF protein levels in neurodegenerative disorders.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Klinisk laboratoriemedicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Clinical Laboratory Medicine (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Biomedicinsk laboratorievetenskap/teknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Biomedical Laboratory Science/Technology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Neurovetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Neurosciences (hsv//eng)
Nyckelord
- AD
- Alzheimer's disease
- Biomarkers
- Cerebrospinal fluid
- Parallel reaction monitoring (PRM)
- Suspension bead array (SBA)
- alpha 1 aantitrypsin
- alpha 1 antichymotrypsin
- apolipoprotein
- biological marker
- cathepsin D
- cholecystokinin
- creatine kinase B type
- dickkopf related protein 3
- fibrinogen alpha
- fructose bisphosphate aldolase C
- glucose regulated protein 94
- inter alpha trypsin inhibitor heavy chain H1
- leucine rich alpha 2 glycoprotein
- neurobeachin
- neurofilament medium polypeptide
- neuromodulin
- plasminogen
- prosaposin
- protein S100B
- SPARC like protein 1
- unclassified drug
- vascular cell adhesion protein 1
- adult
- aged
- Alzheimer disease
- Article
- clinical article
- cohort analysis
- controlled study
- correlational study
- disease course
- female
- human
- male
- mass spectrometry
- middle aged
- mild cognitive impairment
- multiple reaction monitoring
- priority journal
- protein blood level
- protein cerebrospinal fluid level
- protein microarray
- suspension bead array
- very elderly
- AD
- Alzheimer's disease
- Biomarkers
- Cerebrospinal fluid
- Parallel reaction monitoring (PRM)
- Suspension bead array (SBA)
- alpha 1 aantitrypsin
- alpha 1 antichymotrypsin
- apolipoprotein
- biological marker
- cathepsin D
- cholecystokinin
- creatine kinase B type
- dickkopf related protein 3
- fibrinogen alpha
- fructose bisphosphate aldolase C
- glucose regulated protein 94
- inter alpha trypsin inhibitor heavy chain H1
- leucine rich alpha 2 glycoprotein
- neurobeachin
- neurofilament medium polypeptide
- neuromodulin
- plasminogen
- prosaposin
- protein S100B
- SPARC like protein 1
- unclassified drug
- vascular cell adhesion protein 1
- adult
- aged
- Alzheimer disease
- Article
- clinical article
- cohort analysis
- controlled study
- correlational study
- disease course
- female
- human
- male
- mass spectrometry
- middle aged
- mild cognitive impairment
- multiple reaction monitoring
- priority journal
- protein blood level
- protein cerebrospinal fluid level
- protein microarray
- suspension bead array
- very elderly
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas
- Av författaren/redakt...
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Andersson, Annik ...
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Remnestål, Julia
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Nellgård, Bengt, ...
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Vunk, Helian
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Kotol, David
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Edfors, Fredrik
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visa fler...
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Uhlén, Mathias
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Schwenk, Jochen ...
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Ilag, Leopold L.
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Zetterberg, Henr ...
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Blennow, Kaj, 19 ...
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Månberg, Anna, 1 ...
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Nilsson, Peter
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Fredolini, Claud ...
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- Om ämnet
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Medicinsk biotek ...
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Klinisk medicin
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och Klinisk laborato ...
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Medicinsk biotek ...
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och Biomedicinsk lab ...
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Medicinska och f ...
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och Neurovetenskaper
- Artiklar i publikationen
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Clinica Chimica ...
- Av lärosätet
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Kungliga Tekniska Högskolan
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Stockholms universitet
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Göteborgs universitet