Heterobasidion-growth inhibiting Bacillus subtilis A18 exhibits medium- and age-dependent production of lipopeptides

Azeem, MuhammadKTH,Kemi,Department of Chemistry, COMSATS University Islamabad, Abbottabad Campus, Abbottabad 22060, Pakistan,KTH Royal Inst Technol, Sch Engn Sci Chem Biotechnol & Hlth, Dept Chem, SE-10044 Stockholm, Sweden.;COMSATS Univ Islamabad, Dept Chem, Abbottabad Campus, Abbottabad 22060, Pakistan. (författare)

QC 20190715
Heterobasidion annosum s.s. and H. parviporum are severe pathogens of conifers causing butt rot and root rot thus reducing the economic value of timber. Here, the antifungal activity of Bacillus subtilis isolate A18 against these two Heterobasidion species was investigated. Five different culture media with different culture age were investigated to study the effect of substrate composition and culture age for metabolite production. Bacterial cultures and cell-free culture filtrates were tested for antifungal activity. Inhibition of fungal growth was analysed using the agar disc-diffusion method. MALDI-TOF and LC-HRMS analyses were used to identify the antifungal metabolites. Substrate composition and age of culture were found to be active variables with direct effect on the antifungal activity of bacterial culture extracts. High anti-fungal activity was observed when B. subtilis was cultured in PDB, SGB and LB media for four days. Mass-spectrometry analysis showed the presence of lipopeptides in culture filtrates identified as members of the surfactins, polymixins, kurstakins and fengycins. A culture filtrate containing fengycin-type lipopeptides showed the highest bioactivity against Heterobasidion species. Bacterial cultures had higher bioactivity compared to their respective cell free culture filtrates. The results of the present study suggest that B. subtilis A18 is a powerful biocontrol agent against Heterobasidion infections of tree wounds and stumps.

Barba Aliaga, MarinaKTH,Industriell bioteknologi,KTH Royal Inst Technol, Sch Engn Sci Chem Biotechnol & Hlth, Dept Ind Biotechnol, SE-10044 Stockholm, Sweden.(Swepub:kth)u1ltgude (författare)
Borg-Karlson, Anna-KarinKTH,Organisk kemi,Division of Organic Chemistry, Institute of Technology, Tartu University, Tartu 50411, Estonia,KTH Royal Inst Technol, Sch Engn Sci Chem Biotechnol & Hlth, Dept Chem, SE-10044 Stockholm, Sweden.;Tartu Univ, Inst Technol, Div Organ Chem, EE-50411 Tartu, Estonia.(Swepub:kth)u1q875ig (författare)
Terenius, OlleSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Uppsala universitet,Mikrobiologi,Swedish Univ Agr Sci SLU, Dept Ecol, Box 7044, SE-75007 Uppsala, Sweden,Institutionen för ekologi,Department of Ecology,Uppsala University(Swepub:slu)47992 (författare)
Broberg, A.Swedish Univ Agr Sci SLU, Uppsala BioCtr, Dept Mol Sci, Box 7015, SE-75007 Uppsala, Sweden. (författare)
Rajarao, Gunaratna KuttuvaKTH,Industriell bioteknologi,KTH Royal Inst Technol, Sch Engn Sci Chem Biotechnol & Hlth, Dept Ind Biotechnol, SE-10044 Stockholm, Sweden.(Swepub:kth)u12ci1fh (författare)
Broberg, HenrikSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för Molekylära vetenskaper,Department of Molecular Sciences (författare)
KTHKemi (creator_code:org_t)
Sveriges lantbruksuniversitet

Av författaren/redakt...: Azeem, Muhammad; Barba Aliaga, Ma ...; Borg-Karlson, An ...; Terenius, Olle; Broberg, A.; Rajarao, Gunarat ...; visa fler...; Broberg, Henrik; visa färre...

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