Spatial proteomics : a powerful discovery tool for cell biology

• Protein subcellular localization is tightly controlled and intimately linked to protein function in health and disease. Capturing the spatial proteome - that is, the localizations of proteins and their dynamics at the subcellular level - is therefore essential for a complete understanding of cell biology. Owing to substantial advances in microscopy, mass spectrometry and machine learning applications for data analysis, the field is now mature for proteome-wide investigations of spatial cellular regulation. Studies of the human proteome have begun to reveal a complex architecture, including single-cell variations, dynamic protein translocations, changing interaction networks and proteins localizing to multiple compartments. Furthermore, several studies have successfully harnessed the power of comparative spatial proteomics as a discovery tool to unravel disease mechanisms. We are at the beginning of an era in which spatial proteomics finally integrates with cell biology and medical research, thereby paving the way for unbiased systems-level insights into cellular processes. Here, we discuss current methods for spatial proteomics using imaging or mass spectrometry and specifically highlight global comparative applications. The aim of this Review is to survey the state of the field and also to encourage more cell biologists to apply spatial proteomics approaches.

Ämnesord

NATURVETENSKAP  -- Biologi (hsv//swe)

NATURAL SCIENCES  -- Biological Sciences (hsv//eng)

Nyckelord

DUVE C

1964

JOURNAL OF THEORETICAL BIOLOGY

V6

P33 13

NAT METHODS

V10

P315 11

NAT CELL BIOL

V13

P331 18

MOL CELL

V69

P517 mon A. A

2018

17

CELL

V171

P133 17

NATURE

V546

P431 16

NAT METHODS

V13

P837 16

TRENDS CELL BIOL

V26

P804 nworth Marcellus J

2018

Small GTPases

V9

P158 ll Sophia

2017

NATURE COMMUNICATIONS

V8

n Shuo

2018

CURRENT OPINION IN NEUROBIOLOGY

V50

P17 sp Fabian

2017

NEURON

V96

P558 hl Steffen J.

2017

NATURE REVIEWS MOLECULAR CELL BIOLOGY

V18

P685 berts Brock

2017

MOLECULAR BIOLOGY OF THE CELL

V28

P2854 zhak Daniel N.

2017

CELL REPORTS

V20

P2706 icedo Juan C.

2017

NATURE METHODS

V14

P849 ng Siyu

2017

NATURE COMMUNICATIONS

V8

len Mathias

2017

SCIENCE

V357

P660 18

NAT PROTOC

V13

P1445 zal F. M

2018

lenberg J

2018

16

CELL SYST

V3

P361 eiroz R. M. L

2018

15

NAT NEUROSCI

V18

P1819

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