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A High-throughput B...
A High-throughput Bead-based Affinity Assay Enables Analysis of Genital Protein Signatures in Women At Risk of HIV Infection
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- Häggmark, Anna, 1985- (författare)
- KTH,Science for Life Laboratory, SciLifeLab,Affinitets-proteomik
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- Bradley, Frideborg (författare)
- Karolinska Institutet
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- Qundos, Ulrika (författare)
- KTH,Science for Life Laboratory, SciLifeLab,Affinitets-proteomik,KTH Royal Inst Technol, Dept Prot Sci, SciLifeLab, Affin Prote, Stockholm, Sweden
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- Guthrie, Brandon L. (författare)
- Univ Washington, Dept Global Hlth, Washington, DC USA.;Univ Washington, Dept Epidemiol Hlth, Washington, DC USA.
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- Birse, Kenzie (författare)
- Univ Manitoba, Dept Med Microbiol, Winnipeg, MB, Canada.;Publ Hlth Agcy Canada, JC Wilt Infect Dis Ctr, Natl HIV & Retrovirol Labs, Winnipeg, MB, Canada.
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- Noel-Romas, Laura (författare)
- Univ Manitoba, Dept Med Microbiol, Winnipeg, MB, Canada.;Publ Hlth Agcy Canada, JC Wilt Infect Dis Ctr, Natl HIV & Retrovirol Labs, Winnipeg, MB, Canada.
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- Lindskog, Cecilia (författare)
- Uppsala universitet,Science for Life Laboratory, SciLifeLab,Klinisk och experimentell patologi
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- Bosire, Rose (författare)
- Kenya Govt Med Res Ctr, Nairobi, Kenya.
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- Kiarie, James (författare)
- Univ Nairobi, Dept Obstet & Gynecol, Nairobi, Kenya.
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- Farquhar, Carey (författare)
- Univ Washington, Dept Med Global Hlth & Epidemiol, Seattle, WA 98195 USA.
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- Burgener, Adam D. (författare)
- Karolinska Institutet
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- Nilsson, Peter (författare)
- KTH,Science for Life Laboratory, SciLifeLab,Affinitets-proteomik,KTH Royal Inst Technol, Dept Prot Sci, SciLifeLab, Affin Prote, Stockholm, Sweden
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- Broliden, Kristina (författare)
- Karolinska Institutet
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- Månberg, Anna (författare)
- KTH Royal Inst Technol, Dept Prot Sci, SciLifeLab, Affin Prote, Stockholm, Sweden
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(creator_code:org_t)
- AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC, 2019
- 2019
- Engelska.
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Ingår i: Molecular & Cellular Proteomics. - : AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC. - 1535-9476 .- 1535-9484. ; 18:3, s. 461-476
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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https://urn.kb.se/re...
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http://kipublication...
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Abstract
Ämnesord
Stäng
- Women at high risk of HIV infection, including sex workers and those with active genital inflammation, have molecular signatures of immune activation and epithelial barrier remodeling in samples of their genital mucosa. These alterations in the local immunological milieu are likely to impact HIV susceptibility. We here analyze host genital protein signatures in HIV uninfected women, with high frequency of condom use, living in HIV-serodiscordant relationships. Cervicovaginal secretions from women living in HIV-serodiscordant relationships (n = 62) were collected at three time points over 12 months. Women living in HIV-negative seroconcordant relationships (controls, n = 25) were sampled at one time point. All study subjects were examined for demographic parameters associated with susceptibility to HIV infection. The cervicovaginal samples were analyzed using a high-throughput bead-based affinity assay. Proteins involved in epithelial barrier function and inflammation were increased in HIV-serodiscordant women. By combining several methods of analysis, a total of five proteins (CAPG, KLK10, SPRR3, elafin/PI3, CSTB) were consistently associated with this study group. Proteins analyzed using the affinity set-up were further validated by label-free tandem mass spectrometry in a partially overlapping cohort with concordant results. Women living in HIV-serodiscordant relationships thus had elevated levels of proteins involved in epithelial barrier function and inflammation despite low prevalence of sexually transmitted infections and a high frequency of safe sex practices. The identified proteins are important markers to follow during assessment of mucosal HIV susceptibility factors and a high-throughput bead-based affinity set-up could be a suitable method for such evaluation.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Infektionsmedicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Infectious Medicine (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Mikrobiologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Microbiology in the medical area (hsv//eng)
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Häggmark, Anna, ...
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Bradley, Fridebo ...
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Qundos, Ulrika
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Guthrie, Brandon ...
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Birse, Kenzie
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Noel-Romas, Laur ...
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visa fler...
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Lindskog, Cecili ...
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Bosire, Rose
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Kiarie, James
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Farquhar, Carey
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Burgener, Adam D ...
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Nilsson, Peter
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Broliden, Kristi ...
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Månberg, Anna
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MEDICIN OCH HÄLS ...
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och Medicinska och f ...
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Klinisk medicin
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och Infektionsmedici ...
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Medicinska och f ...
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och Mikrobiologi ino ...
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Molecular & Cell ...
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Kungliga Tekniska Högskolan
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Uppsala universitet
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Karolinska Institutet