A rapid smartphone-based lactate dehydrogenase test for neonatal diagnostics at the point of care

Halvorsen, Cecilia PegelowKarolinska Institutet,Karolinska Inst, Dept Clin Res & Educ, Sodersjukhuset, Stockholm, Sweden;Sachs Children & Youth Hosp, Neonatal Unit, Stockholm, Sweden (författare)

QC 20190731
There is a growing recognition of the importance of point-of-care tests (POCTs) for detecting critical neonatal illnesses to reduce the mortality rate in newborns, especially in low-income countries, which account for 98 percent of reported neonatal deaths. Lactate dehydrogenase (LDH) is a marker of cellular damage as a result of hypoxia-ischemia in affected organs. Here, we describe and test a POC LDH test direct from whole blood to provide early indication of serious illness in the neonate. The sample-inresult- out POC platform is specifically designed to meet the needs at resource-limited settings. Plasma is separated from whole blood on filter paper with dried-down reagents for colorimetric reaction, combined with software for analysis using a smartphone. The method was clinically tested in newborns in two different settings. In a clinical cohort of newborns of Stockholm (n = 62) and Hanoi (n = 26), the value of R using Pearson's correlation test was 0.91 (p < 0.01) and the R-2 = 0.83 between the two methods. The mean LDH (+/- SD) for the reference method vs. the POC-LDH was 551 (+/- 280) U/L and 552 (+/- 249) U/L respectively, indicating the clinical value of LDH values measured in minutes with the POC was comparable with standardized laboratory analyses.

Olson, LinusKarolinska Institutet,Karolinska Inst, Dept Womens & Childrens Hlth, Stockholm, Sweden;Karolinska Inst, Dept Publ Hlth Sci, Stockholm, Sweden;TRAC Sweden Vietnam, Hanoi, Vietnam (författare)
Araujo, Ana CatarinaCalmark Sweden AB, Stockholm, Sweden. (författare)
Karlsson, MathiasUppsala universitet,Klinisk kemi,Calmark Sweden AB, Stockholm, Sweden(Swepub:uu)matka447 (författare)
Nguyen, Trang ThiVietnam Natl Childrens Hosp, Neonatal Intens Care Unit, Hanoi, Vietnam;Res Inst Child Hlth, Hanoi, Vietnam (författare)
Khu, Dung T. K.TRAC Sweden Vietnam, Hanoi, Vietnam.;Vietnam Natl Childrens Hosp, Neonatal Intens Care Unit, Hanoi, Vietnam. (författare)
Le, Ha T. T.Vietnam Natl Childrens Hosp, Neonatal Intens Care Unit, Hanoi, Vietnam.;Res Inst Child Hlth, Hanoi, Vietnam. (författare)
Nguyen, Hoa T. B.Vietnam Natl Childrens Hosp, Neonatal Intens Care Unit, Hanoi, Vietnam.;Res Inst Child Hlth, Hanoi, Vietnam. (författare)
Winbladh, BirgerKarolinska Inst, Dept Clin Res & Educ, Sodersjukhuset, Stockholm, Sweden. (författare)
Russom, Aman,Assoc.Prof.KTH,Proteinvetenskap,Science for Life Laboratory, SciLifeLab,KTH Royal Inst Technol, Div Nanobiotechnol, Dept Prot Sci, Sch Engn Sci Chem Biotechnol & Hlth,Sci Life Lab, Stockholm, Sweden(Swepub:kth)u1go6jxm (författare)
Karolinska InstitutetKarolinska Inst, Dept Clin Res & Educ, Sodersjukhuset, Stockholm, Sweden;Sachs Children & Youth Hosp, Neonatal Unit, Stockholm, Sweden (creator_code:org_t)

Av författaren/redakt...: Halvorsen, Cecil ...; Olson, Linus; Araujo, Ana Cata ...; Karlsson, Mathia ...; Nguyen, Trang Th ...; Khu, Dung T. K.; visa fler...; Le, Ha T. T.; Nguyen, Hoa T. B ...; Winbladh, Birger; Russom, Aman, As ...; visa färre...

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Av lärosätet: Kungliga Tekniska Högskolan; Karolinska Institutet; Uppsala universitet

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