onr:"swepub:oai:DiVA.org:kth-26071"
Sökning: onr:"swepub:oai:DiVA.org:kth-26071" > Electrospray ioniza...
- 1 av 1
- Föregående post
- Nästa post
- Till träfflistan
| Fältnamn | Indikatorer | Metadata |
|---|---|---|
| 000 | 02630naa a2200373 4500 | |
| 001 | oai:DiVA.org:kth-26071 | |
| 003 | SwePub | |
| 008 | 101111s2010 | |||||||||||000 ||eng| | |
| 024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-260712 URI |
| 024 | 7 | a https://doi.org/10.1002/rcm.46742 DOI |
| 040 | a (SwePub)kth | |
| 041 | a engb eng | |
| 042 | 9 SwePub | |
| 072 | 7 | a ref2 swepub-contenttype |
| 072 | 7 | a art2 swepub-publicationtype |
| 100 | 1 | a Ek, Patriku KTH,Analytisk kemi4 aut0 (Swepub:kth)u1rk4org |
| 245 | 1 0 | a Electrospray ionization mass spectrometry from discrete nanoliter-sized sample volumes |
| 264 | c 2010-07-27 | |
| 264 | 1 | b Wiley,c 2010 |
| 338 | a print2 rdacarrier | |
| 500 | a QC 20101111 | |
| 520 | a We describe a method for nanoelectrospray ionization mass spectrometry (nESI-MS) of very small sample volumes. Nanoliter-sized sample droplets were taken up by suction into a nanoelectrospray needle from a silicon microchip prior to ESI. To avoid a rapid evaporation of the small sample volumes, all manipulation steps were performed under a cover of fluorocarbon liquid. Sample volumes down to 1.5 nL were successfully analyzed, and an absolute limit of detection of 105 attomole of insulin (chain B, oxidized) was obtained. The open access to the sample droplets on the silicon chip provides the possibility to add reagents to the sample droplets and perform chemical reactions under an extended period of time. This was demonstrated in an example where we performed a tryptic digestion of cytochrome C in a nanoliter-sized sample volume for 2.5h, followed by monitoring the outcome of the reaction with nESI-MS. The technology was also utilized for tandem mass spectrometry (MS/MS) sequencing analysis of a 2 nL solution of angiotensin I. | |
| 650 | 7 | a NATURVETENSKAPx Kemix Analytisk kemi0 (SwePub)104012 hsv//swe |
| 650 | 7 | a NATURAL SCIENCESx Chemical Sciencesx Analytical Chemistry0 (SwePub)104012 hsv//eng |
| 653 | a Analytical chemistry | |
| 653 | a Analytisk kemi | |
| 700 | 1 | a Stjernström, Mårtenu KTH,Analytisk kemi4 aut0 (Swepub:kth)u11asu0n |
| 700 | 1 | a Emmer, Åsau KTH,Analytisk kemi4 aut0 (Swepub:kth)u1czoysx |
| 700 | 1 | a Roeraade, Johanu KTH,Analytisk kemi4 aut0 (Swepub:kth)u10i2qgl |
| 710 | 2 | a KTHb Analytisk kemi4 org |
| 773 | 0 | t Rapid Communications in Mass Spectrometryd : Wileyg 24:17, s. 2561-2568q 24:17<2561-2568x 0951-4198x 1097-0231 |
| 856 | 4 | u https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/rcm.4674 |
| 856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-26071 |
| 856 | 4 8 | u https://doi.org/10.1002/rcm.4674 |
Hitta via bibliotek
- Rapid Communications in Mass Spectrometry (Sök värdpublikationen i LIBRIS)
Till lärosätets databas
- 1 av 1
- Föregående post
- Nästa post
- Till träfflistan
Hitta mer i SwePub
- Av författaren/redakt...
- Ek, Patrik
- Stjernström, Mår ...
- Emmer, Åsa
- Roeraade, Johan
- Om ämnet
-
- NATURVETENSKAP
- NATURVETENSKAP
- och Kemi
- och Analytisk kemi
- Artiklar i publikationen
- Rapid Communicat ...
- Av lärosätet
- Kungliga Tekniska Högskolan
Sök utanför SwePub
- Sök vidare i:
- Google Book Search
- Google Scholar
Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.
- Copyright © LIBRIS - Nationella bibliotekssystem
- LIBRIS.kb.se
