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High-definition spatial transcriptomics for in situ tissue profiling

Vickovic, Sanja (author)
KTH Royal Institute of Technology,KTH,Science for Life Laboratory, SciLifeLab,Genteknologi,Broad Inst MIT & Harvard, Klarman Cell Observ, Cambridge, MA 02142 USA.,Broad Institute
Eraslan, Gokcen (author)
Broad Inst MIT & Harvard, Klarman Cell Observ, Cambridge, MA 02142 USA.,Broad Institute
Salmén, Fredrik (author)
KTH Royal Institute of Technology,KTH,Genteknologi,Science for Life Laboratory, SciLifeLab
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Klughammer, Johanna (author)
Broad Inst MIT & Harvard, Klarman Cell Observ, Cambridge, MA 02142 USA.,Broad Institute
Stenbeck, Linnea (author)
KTH Royal Institute of Technology,KTH,Science for Life Laboratory, SciLifeLab,Genteknologi
Schapiro, Denis (author)
Broad Inst MIT & Harvard, Klarman Cell Observ, Cambridge, MA 02142 USA.;Harvard Med Sch, Lab Syst Pharmacol, Boston, MA 02115 USA.,Broad Institute,Harvard University
Aijo, Tarmo (author)
Flatiron Inst, Ctr Computat Biol, New York, NY USA.,Flatiron Institute
Bonneau, Richard (author)
NYU, Ctr Data Sci, New York, NY USA.;Brigham & Womens Hosp, Dept Pathol, 75 Francis St, Boston, MA 02115 USA.,Brigham and Women's Hospital / Harvard Medical School,New York University
Bergenstrahle, Joseph (author)
KTH,Genteknologi,Science for Life Laboratory, SciLifeLab
Fernandez Navarro, Jose (author)
KTH Royal Institute of Technology,KTH,Genteknologi,Science for Life Laboratory, SciLifeLab
Gould, Joshua (author)
Broad Inst MIT & Harvard, Klarman Cell Observ, Cambridge, MA 02142 USA.,Broad Institute
Griffin, Gabriel K. (author)
Broad Inst MIT & Harvard, Klarman Cell Observ, Cambridge, MA 02142 USA.;Brigham & Womens Hosp, Dept Pathol, 75 Francis St, Boston, MA 02115 USA.,Brigham and Women's Hospital / Harvard Medical School,Broad Institute
Borg, Åke (author)
Lund University,Lunds universitet,Familjär bröstcancer,Forskargrupper vid Lunds universitet,Familial Breast Cancer,Lund University Research Groups
Ronaghi, Mostafa (author)
Illumina Inc, San Diego, CA USA.
Frisen, Jonas (author)
Karolinska Institutet,Karolinska Institute
Lundeberg, Joakim (author)
KTH Royal Institute of Technology,KTH,Science for Life Laboratory, SciLifeLab,Genteknologi,Stanford Univ, Dept Bioengn, Stanford, CA 94305 USA.,Stanford University
Regev, Aviv (author)
Broad Inst MIT & Harvard, Klarman Cell Observ, Cambridge, MA 02142 USA.;MIT, Howard Hughes Med Inst, Cambridge, MA USA.;MIT, Dept Biol, Koch Inst Integrat Canc Res, Cambridge, MA USA.,Broad Institute,Massachusetts Institute of Technology
Ståhl, Patrik, Dr. (author)
KTH Royal Institute of Technology,KTH,Science for Life Laboratory, SciLifeLab,Genteknologi
Bergenstråhle, Ludvig (author)
KTH Royal Institute of Technology
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 (creator_code:org_t)
2019-09-09
2019
English.
In: Nature Methods. - : NATURE PUBLISHING GROUP. - 1548-7091 .- 1548-7105. ; 16:10, s. 987-
  • Journal article (peer-reviewed)
Abstract Subject headings
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  • Spatial and molecular characteristics determine tissue function, yet high-resolution methods to capture both concurrently are lacking. Here, we developed high-definition spatial transcriptomics, which captures RNA from histological tissue sections on a dense, spatially barcoded bead array. Each experiment recovers several hundred thousand transcriptcoupled spatial barcodes at 2-mu m resolution, as demonstrated in mouse brain and primary breast cancer. This opens the way to high-resolution spatial analysis of cells and tissues.

Subject headings

NATURVETENSKAP  -- Biologi (hsv//swe)
NATURAL SCIENCES  -- Biological Sciences (hsv//eng)
TEKNIK OCH TEKNOLOGIER  -- Medicinteknik -- Medicinsk laboratorie- och mätteknik (hsv//swe)
ENGINEERING AND TECHNOLOGY  -- Medical Engineering -- Medical Laboratory and Measurements Technologies (hsv//eng)

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