Development of a tyrosine-tyrosinase it for connecting cellular communication with electronic networks

QC 20191115
There is a growing interest in mediating information transfer between biology and electronics. By the addition of redox mediators to various samples, one can electronically obtain a redox “portrait” of a biological system and additionally program gene expression in suitably engineered cells. We have created a cell-based synthetic biology-electrochemical axis in which engineered cells process molecular cues producing an output that can be directly recorded via electronics – without added redox mediators. The process is robust; two key components must be together to provide a valid signal. The system builds on the tyrosinase-mediated conversion of tyrosine to L-DOPA and L-DOPAquinone, which are both redox active. “Sensor” cells provide for signal-mediated surface expression of tyrosinase. Similarly, “producer” cells synthesize and export tyrosine. In co-cultures, this system enables real-time electrochemical transduction of the original molecular cues. To demonstrate, we eavesdrop on the quorum sensing molecules from Pseudomonas aeruginosa N-(3-oxododecanoyl)-l-homoserine lactone and pyocyanin.

Hörnström, DavidKTH,Industriell bioteknologi(Swepub:kth)u1265sok (författare)
Sjöberg, GustavKTH,Industriell bioteknologi(Swepub:kth)u1epvtpk (författare)
Järbur, Ida (författare)
Payne, Gregory F. (författare)
Van Maris, Antonius J.KTH,Industriell bioteknologi(Swepub:kth)u1n330r1 (författare)
Bentley, William E. (författare)
KTHIndustriell bioteknologi (creator_code:org_t)

Av författaren/redakt...: VanArsdale, Eric; Hörnström, David; Sjöberg, Gustav; Järbur, Ida; Payne, Gregory F ...; Van Maris, Anton ...; visa fler...; Bentley, William ...; visa färre...

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