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A high-resolution spatial map of cilia-associated proteins based on characterization of the human fallopian tube-specific proteome

Hikmet Noraddin, Feria, 1989- (författare)
Uppsala universitet,Cancerprecisionsmedicin,Cecilia Lindskog/Human Protein Atlas
Digre, Andreas (författare)
Uppsala universitet,Cancerprecisionsmedicin,Cecilia Lindskog/Human Protein Atlas
Hansen, Jan Niklas (författare)
Science for Life Laboratory, School of Engineering Sciences in Chemistry, Biotechnology and Health, KTH Royal Institute of Technology, 17121 Stockholm, Sweden; Department of Bioengineering, Stanford University, Stanford, CA 94305, USA
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Schutten, Rutger (författare)
Uppsala universitet,Cancerprecisionsmedicin
Lundberg, Emma (författare)
Science for Life Laboratory, School of Engineering Sciences in Chemistry, Biotechnology and Health, KTH Royal Institute of Technology, 17121 Stockholm, Sweden; Department of Bioengineering, Stanford University, Stanford, CA 94305, USA; Department of Pathology, Stanford University, Stanford, CA 94305, USA; Chan Zuckerberg Biohub, San Francisco, San Francisco, CA 94158, USA
Uhlén, Mathias (författare)
Science for Life Laboratory, School of Engineering Sciences in Chemistry, Biotechnology and Health, KTH Royal Institute of Technology, 17121 Stockholm, Sweden; Department of Neuroscience, Karolinska Institutet, Stockholm, Sweden
Méar, Loren (författare)
Uppsala universitet,Cancerprecisionsmedicin,Division of Obstetrics and Gynecology, Department of Clinical Science, Intervention and Technology, Karolinska Institutet and Karolinska University Hospital, SE-14186 Stockholm, Sweden,Cecilia Lindskog/Human Protein Atlas
Lindskog, Cecilia (författare)
Uppsala universitet,Cancerprecisionsmedicin,Cecilia Lindskog/Human Protein Atlas
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 (creator_code:org_t)
Engelska.
Relaterad länk:
https://urn.kb.se/re...
  • Annan publikation (övrigt vetenskapligt/konstnärligt)
Abstract Ämnesord
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  • Molecular changes in the fallopian tubes (FT) play a crucial role in the development of cancer and reproductive disorders. Here, we aimed to map key FT proteins on the single-cell level utilizing an integrated transcriptomics and proteomics approach. Based on RNA-seq, 310 genes were identified as elevated in FT, out of which a majority were associated with motile cilia function. An in-depth spatial characterization was performed for 133 of these genes in FT and other human tissues with motile cilia, localizing the proteins to different subcellular structures of ciliated cells. The specificity for ciliated cells was validated with single-cell RNA-seq and spatial mass-spectrometry data. Our approach enabled us to identify 44 novel cilia-related proteins lacking previous evidence on the protein level, as well as several other proteins not described in the context of cilia biology. The high-resolution spatial map aids in further disentangling pathways involved in infertility and diseases linked to cilia-specific functions.

Nyckelord

motile cilia
fallopian tube
transcriptomics
spatial proteomics

Publikations- och innehållstyp

vet (ämneskategori)
ovr (ämneskategori)

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