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Optimization study ...
Optimization study on periodic counter-current chromatography integrated in a monoclonal antibody downstream process
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- Gomis-Fons, Joaquin (författare)
- KTH Royal Institute of Technology,Lund University,Lunds universitet,KTH,Centre for Advanced BioProduction by Continuous Processing, AdBIOPRO,ept. of Chemical Engineering, Lund University, Lund, Sweden,Avdelningen för kemiteknik,Institutionen för processteknik och tillämpad biovetenskap,Institutioner vid LTH,Lunds Tekniska Högskola,Division of Chemical Engineering,Department of Process and Life Science Engineering,Departments at LTH,Faculty of Engineering, LTH
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- Andersson, Niklas (författare)
- Lund University,Lunds universitet,Avdelningen för kemiteknik,Institutionen för processteknik och tillämpad biovetenskap,Institutioner vid LTH,Lunds Tekniska Högskola,Division of Chemical Engineering,Department of Process and Life Science Engineering,Departments at LTH,Faculty of Engineering, LTH
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- Nilsson, Bernt (författare)
- KTH Royal Institute of Technology,Lund University,Lunds universitet,KTH,Centre for Advanced BioProduction by Continuous Processing, AdBIOPRO,Dept. of Chemical Engineering, Lund University, Lund, Sweden,Avdelningen för kemiteknik,Institutionen för processteknik och tillämpad biovetenskap,Institutioner vid LTH,Lunds Tekniska Högskola,Division of Chemical Engineering,Department of Process and Life Science Engineering,Departments at LTH,Faculty of Engineering, LTH
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(creator_code:org_t)
- Elsevier, 2020
- 2020
- Engelska.
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Ingår i: Journal of Chromatography A. - : Elsevier. - 0021-9673 .- 1873-3778. ; 1621
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Abstract
Ämnesord
Stäng
- An optimization study of an integrated periodic counter-current chromatography (PCC) process in a monoclonal antibody (mAb) downstream process at lab scale, is presented in this paper. The optimization was based on a mechanistic model of the breakthrough curve in the protein-A capture step. Productivity and resin utilization were the objective functions, while yield during the loading of the capture column was set as a constraint. Different integration approaches were considered, and the effect of the feed concentration, yield and the protein-A resin was studied. The breakthrough curve and the length of the product recovery, which depended on the integration approach, determined the process scheduling. Several optimal Pareto solutions were obtained. At 0.5 mg mL−1 and 99% yield, a maximum productivity of 0.38 mg mL−1 min−1 with a resin utilization of 60% was obtained. On the other hand, the maximum resin utilization was 95% with a productivity of 0.10 mg mL−1 min−1. Due to the constraint of the process scheduling, a lower productivity could be achieved in the integration approaches with higher recovery time, which was more remarkable at higher concentrations. Therefore, it was shown that a holistic approach, where all the purification steps are considered in the process optimization, is needed to design a PCC in a downstream process.
Ämnesord
- TEKNIK OCH TEKNOLOGIER -- Kemiteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Chemical Engineering (hsv//eng)
- TEKNIK OCH TEKNOLOGIER -- Kemiteknik -- Kemiska processer (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Chemical Engineering -- Chemical Process Engineering (hsv//eng)
Nyckelord
- Downstream processing
- Monoclonal antibody purification
- Periodic counter-current chromatography
- Process integration
- Process optimization
- Downstream processing
- Monoclonal antibody purification
- Periodic counter-current chromatography
- Process integration
- Process optimization
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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