The Antibody Society's antibody validation webinar series

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QC 20200825
In the wake of the reproducibility crisis and numerous discussions on how commercially available antibodies as research tool contribute to it, The Antibody Society developed a series of 10 webinars to address the issues involved. The webinars were delivered by speakers with both academic and commercial backgrounds. This report highlights the problems, and offers solutions to help the scientific community appropriately identify the right antibodies and to validate them for their research and development projects. Despite the various solutions proposed here, they must be applied on a case-by-case basis. Each antibody must be verified based on the content of the product sheet, and subsequently through experimentation to confirm integrity, specificity and selectivity. Verification needs to focus on the precise application and tissue/cell type for which the antibody will be used, and all verification data must be reported openly. The various approaches discussed here all have caveats, so a combination of solutions must be considered.

MEDICIN OCH HÄLSOVETENSKAP Medicinsk bioteknologi Medicinsk bioteknologi hsv//swe
MEDICAL AND HEALTH SCIENCES Medical Biotechnology Medical Biotechnology hsv//eng
Antibody specificity
antibody selectivity
specific antibodies
selective antibodies
reproducibility
recombinant antibodies
monoclonal antibodies
polyclonal antibodies
peptide antibodies
antibody validation

Bandrowski, AnitaUniv Calif San Diego, Ctr Res Biol Syst, La Jolla, CA 92093 USA. (författare)
Begley, C. GlennBioCurate, Parkville, Vic, Australia. (författare)
Bradbury, Andrew R. M.Specifica Inc, Santa Fe, NM USA. (författare)
Chalmers, Andrew D.Univ Bath, Dept Biol & Biochem, Bath, Avon, England.;CiteAb, Bath, Avon, England. (författare)
Gomes, Aldrin V.Univ Calif Davis, Dept Neurobiol Physiol & Behav, Davis, CA 95616 USA. (författare)
Hardcastle, TravisWaterbeach, Horizon Discovery, Cambridge, England. (författare)
Lund-Johansen, FridtjofOslo Univ Hosp, Dept Immunol, Oslo, Norway. (författare)
Plueckthun, AndreasUniv Zurich, Dept Biochem, Zurich, Switzerland. (författare)
Roncador, GiovannaSpanish Natl Canc Res Ctr, Monoclonal Antibody Unit, Madrid, Spain. (författare)
Solache, AlejandraAbcam Plc, Discovery Dr,Biomed Campus, Cambridge, England. (författare)
Taussig, Michael J.Cambridge Prot Arrays Ltd, Babraham Res Campus, Cambridge, England. (författare)
Trimmer, James S.Univ Calif Davis, Sch Med, Dept Physiol & Membrane Biol, Davis, CA 95616 USA. (författare)
Williams, Cecilia,Professor,1969-Karolinska Institutet,KTH,Science for Life Laboratory, SciLifeLab,Proteinvetenskap,Karolinska Inst, Dept Biosci & Nutr, Huddinge, Sweden.(Swepub:kth)u1ygqmuy (författare)
Goodman, Simon L.Antibody Soc Inc, Framingham, MA USA. (författare)
Aeonian Biotech Ltd, Marcham OX13 6PH, England.Univ Calif San Diego, Ctr Res Biol Syst, La Jolla, CA 92093 USA. (creator_code:org_t)

Av författaren/redakt...: Voskuil, Jan L. ...; Bandrowski, Anit ...; Begley, C. Glenn; Bradbury, Andrew ...; Chalmers, Andrew ...; Gomes, Aldrin V.; visa fler...; Hardcastle, Trav ...; Lund-Johansen, F ...; Plueckthun, Andr ...; Roncador, Giovan ...; Solache, Alejand ...; Taussig, Michael ...; Trimmer, James S ...; Williams, Cecili ...; Goodman, Simon L ...; visa färre...

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