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Detecting harmful a...
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Toldrà Filella, AnnaKTH,Fiberteknologi,IRTA, Ctra Poble Nou Km 5-5, Tarragona 43540, Spain.
(författare)
Detecting harmful algal blooms with nucleic acid amplification-based biotechnological tools
- Artikel/kapitelEngelska2020
Förlag, utgivningsår, omfång ...
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Elsevier BV,2020
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printrdacarrier
Nummerbeteckningar
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LIBRIS-ID:oai:DiVA.org:kth-287520
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https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-287520URI
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https://doi.org/10.1016/j.scitotenv.2020.141605DOI
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Språk:engelska
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Sammanfattning på:engelska
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Ämneskategori:ref swepub-contenttype
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Ämneskategori:for swepub-publicationtype
Anmärkningar
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QC 20201218
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Harmful algal blooms (HABs) represent a growing threat to aquatic ecosystems and humans. Effective HAB management and mitigation efforts strongly rely on the availability of timely and in-situ tools for the detection of microalgae. In this sense, nudeic acid-based (molecular) methods are being considered for the unequivocal identification of miaoalgae as an attractive alternative to the currently used time-consuming and laboratory-based light microscopy techniques. This review provides an overview of the progress made on new molecular biotechnological tools for microalgal detection, particularly focusing on those that combine a nudeic acid (DNA or RNA) amplification step with detection. Different types of amplification processes (thermal and isothermal) and detection formats (e.g. microarrays, biosensors, lateral flows) are presented, and a comprehensive overview of their advantages and limitations is provided Although isothermal techniques are an attractive alternative to thermal amplification to reach in-situ analysis, further development is still required. Finally, current challenges, critical steps and future directions of the whole analysis process ( from sample procurement to in-situ implementation) are described.
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Biuppslag (personer, institutioner, konferenser, titlar ...)
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O'Sullivan, Ciara K.Univ Rovira & Virgili, Dept Engn Quim, Ave Paisos Catalans 26, Tarragona 43007, Spain.;Inst Catalana Recerca & Estudis Avancats ICREA, Pg Lluis Co 23, Barcelona 08010, Spain.
(författare)
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Diogene, JorgeIRTA, Ctra Poble Nou Km 5-5, Tarragona 43540, Spain.
(författare)
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Campas, MonicaIRTA, Ctra Poble Nou Km 5-5, Tarragona 43540, Spain.
(författare)
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KTHFiberteknologi
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:Science of the Total Environment: Elsevier BV7490048-96971879-1026
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