WFRF:(Cho Nathan H.)
Sökning: WFRF:(Cho Nathan H.) > Spatiotemporal diss...
Spatiotemporal dissection of the cell cycle with single-cell proteogenomics
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- Mahdessian, Diana (författare)
- KTH,Science for Life Laboratory, SciLifeLab,Cellulär och klinisk proteomik,KTH Royal Inst Technol, Sch Engn Sci Chem, Sci Life Lab Biotechnol & Hearth, Stockholm, Sweden.
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- Cesnik, Anthony J. (författare)
- KTH,Science for Life Laboratory, SciLifeLab,Cellulär och klinisk proteomik,KTH Royal Inst Technol, Sch Engn Sci Chem, Sci Life Lab Biotechnol & Hearth, Stockholm, Sweden.;Stanford Univ, Dept Genet, Stanford, CA 94305 USA.;Chan Zuckerberg Biohub, San Francisco, CA 94158 USA.
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- Gnann, Christian (författare)
- KTH,Science for Life Laboratory, SciLifeLab,Cellulär och klinisk proteomik,KTH Royal Inst Technol, Sch Engn Sci Chem, Sci Life Lab Biotechnol & Hearth, Stockholm, Sweden.;Chan Zuckerberg Biohub, San Francisco, CA 94158 USA.
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- 2021-02-24
- 2021
- Engelska.
- Ingår i: Nature. - : Springer Nature. - 0028-0836 .- 1476-4687. ; 590:7847
- Tidskriftsartikel (refereegranskat)
Abstract
Ämnesord
Stäng
- Spatial and temporal variations among individual human cell proteomes are comprehensively mapped across the cell cycle using proteomic imaging and transcriptomics. The cell cycle, over which cells grow and divide, is a fundamental process of life. Its dysregulation has devastating consequences, including cancer(1-3). The cell cycle is driven by precise regulation of proteins in time and space, which creates variability between individual proliferating cells. To our knowledge, no systematic investigations of such cell-to-cell proteomic variability exist. Here we present a comprehensive, spatiotemporal map of human proteomic heterogeneity by integrating proteomics at subcellular resolution with single-cell transcriptomics and precise temporal measurements of individual cells in the cell cycle. We show that around one-fifth of the human proteome displays cell-to-cell variability, identify hundreds of proteins with previously unknown associations with mitosis and the cell cycle, and provide evidence that several of these proteins have oncogenic functions. Our results show that cell cycle progression explains less than half of all cell-to-cell variability, and that most cycling proteins are regulated post-translationally, rather than by transcriptomic cycling. These proteins are disproportionately phosphorylated by kinases that regulate cell fate, whereas non-cycling proteins that vary between cells are more likely to be modified by kinases that regulate metabolism. This spatially resolved proteomic map of the cell cycle is integrated into the Human Protein Atlas and will serve as a resource for accelerating molecular studies of the human cell cycle and cell proliferation.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
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