Genome-wide estrogen receptor β chromatin binding in humancolon cancer cells reveals its tumor suppressor activity

Indukuri, RajithaKarolinska Institutet,KTH,Science for Life Laboratory, SciLifeLab,Cellulär och klinisk proteomik,cali.nuur@indek.kth.se,Cecilia Williams (author)

Colorectal cancer (CRC) is the third leading cause of cancer death in the western world. In women, menopausal hormone therapy has been shown to reduce CRC incidence by 20%. Studies demonstrate that estrogen activating estrogen receptor beta (ERβ) protects against CRC. ERβ is a nuclear receptor that regulates gene expression through interactions with the chromatin. This molecular mechanism is, however, not well characterized in colon. Here, we present for the first time, the cistrome of ERβ in different colon cancer cell lines. We use cell lines engineered to express ERβ, optimize and validate an ERβ antibody for chromatin-immunoprecipitation (ChIP), and perform ChIP-Seq. We identify key binding motifs, including ERE, AP-1, and TCF sites, and we determine enrichment of binding to cis-regulatory chromatin sites of genes involved in tumor development, cell migration, cell adhesion, apoptosis, and Wnt signaling pathways. We compare the corresponding cistromes of colon and breast cancer and find that they are conserved for about a third of genes, including GREB1, but that ERβ tethering to TCF and KLF family motifs is characteristic for colon. We exemplify upregulation of putative CRC tumor suppressor gene CST5 where ERβ in colon cells binds to cis-regulatory regions nearby (−351 bp) the transcriptional start site. Our work provides a foundation for understanding the mechanism of action of ERβ in CRC prevention.

Jafferali, Mohammed HakimKTH,Proteinvetenskap(Swepub:kth)u1kx7gi1 (author)
Song, DandanKarolinska Institutet (author)
Damdimopoulos, AnastasiosKarolinska Institutet (author)
Hases, LinneaKarolinska Institutet,KTH,Science for Life Laboratory, SciLifeLab,Proteinvetenskap,Department of Biosciences and Nutrition, Karolinska Institutet, Huddinge, Swede(Swepub:kth)u1ccjxeo (author)
Zhao, ChunyanDepartment of Biosciences and Nutrition, Karolinska Institutet, Huddinge, Swede (author)
Williams, Cecilia,Professor,1969-Karolinska Institutet,KTH,Science for Life Laboratory, SciLifeLab,Proteinvetenskap,Department of Biosciences and Nutrition, Karolinska Institutet, Huddinge, Swede(Swepub:kth)u1ygqmuy (author)
Archer, AKarolinska Institutet (author)
KTHScience for Life Laboratory, SciLifeLab (creator_code:org_t)

By the author/editor: Indukuri, Rajith ...; Jafferali, Moham ...; Song, Dandan; Damdimopoulos, A ...; Hases, Linnea; Zhao, Chunyan; show more...; Williams, Cecili ...; Archer, A; show less...

About the subject

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Clinical Medicin ...; and Cancer and Oncol ...

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Clinical Medicin ...; and Endocrinology an ...

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