Search: WFRF:(Indukuri Rajitha) >
Estrogen Receptor B...
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Indukuri, RajithaKarolinska Institutet,KTH,Science for Life Laboratory, SciLifeLab,Cellulär och klinisk proteomik,Department of Biosciences and Nutrition, Karolinska Institutet, Huddinge, Sweden,Cecilia Williams
(author)
Estrogen Receptor Beta Influences the Inflammatory p65 Cistrome in Colon Cancer Cells
- Article/chapterEnglish2021
Publisher, publication year, extent ...
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2021-03-30
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FRONTIERS,2021
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electronicrdacarrier
Numbers
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LIBRIS-ID:oai:DiVA.org:kth-293860
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https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-293860URI
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https://doi.org/10.3389/fendo.2021.650625DOI
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http://kipublications.ki.se/Default.aspx?queryparsed=id:146342652URI
Supplementary language notes
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Language:English
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Summary in:English
Part of subdatabase
Classification
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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QC 20210510
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Inflammation is a primary component of both initiation and promotion of colorectal cancer (CRC). Cytokines secreted by macrophages, including tumor necrosis factor alpha (TNFα), activates the pro-survival transcription factor complex NFκB. The precise mechanism of NFκB in CRC is not well studied, but we recently reported the genome-wide transcriptional impact of TNFα in two CRC cell lines. Further, estrogen signaling influences inflammation in a complex manner and suppresses CRC development. CRC protective effects of estrogen have been shown to be mediated by estrogen receptor beta (ERβ, ESR2), which also impacts inflammatory signaling of the colon. However, whether ERβ impacts the chromatin interaction (cistrome) of the main NFκB subunit p65 (RELA) is not known. We used p65 chromatin immunoprecipitation followed by sequencing (ChIP-Seq) in two different CRC cell lines, HT29 and SW480, with and without expression of ERβ. We here present the p65 colon cistrome of these two CRC cell lines. We identify that RELA and AP1 motifs are predominant in both cell lines, and additionally describe both common and cell line-specific p65 binding sites and correlate these to transcriptional changes related to inflammation, migration, apoptosis and circadian rhythm. Further, we determine that ERβ opposes a major fraction of p65 chromatin binding in HT29 cells, but enhances p65 binding in SW480 cells, thereby impacting the p65 cistrome differently in the two cell lines. However, the biological functions of the regulated genes appear to have similar roles in both cell lines. To our knowledge, this is the first time the p65 CRC cistrome is compared between different cell lines and the first time an influence by ERβ on the p65 cistrome is investigated. Our work provides a mechanistic foundation for a better understanding of how estrogen influences inflammatory signaling through NFκB in CRC cells.
Subject headings and genre
Added entries (persons, corporate bodies, meetings, titles ...)
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Hases, LinneaKarolinska Institutet,KTH,Cellulär och klinisk proteomik,Science for Life Laboratory, SciLifeLab,Department of Biosciences and Nutrition, Karolinska Institutet, Huddinge, Sweden,Cecilia Williams(Swepub:kth)u1ccjxeo
(author)
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Archer, AmenaKarolinska Institutet,KTH,Cellulär och klinisk proteomik,Science for Life Laboratory, SciLifeLab,Department of Biosciences and Nutrition, Karolinska Institutet, Huddinge, Sweden(Swepub:kth)u131bapq
(author)
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Williams, Cecilia,Professor,1969-Karolinska Institutet,KTH,Cellulär och klinisk proteomik,Science for Life Laboratory, SciLifeLab,Department of Biosciences and Nutrition, Karolinska Institutet, Huddinge, Sweden(Swepub:kth)u1ygqmuy
(author)
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KTHScience for Life Laboratory, SciLifeLab
(creator_code:org_t)
Related titles
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In:Frontiers in Endocrinology: FRONTIERS12, s. 1-131664-2392
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