Endochondral Ossification Induced by Cell Transplantation of Endothelial Cells and Bone Marrow Stromal Cells with Copolymer Scaffold Using a Rat Calvarial Defect Model

↓ Direkt till sidans innehåll
↓ Direkt till sidans sekundära innehåll (sidomenyn)

Search: id:"swepub:oai:DiVA.org:kth-296195" > Endochondral Ossifi...

1 of 1
Previous record
Next record
To hitlist

Details
MARC

Xing, ZheLanzhou Univ, Sch Stomatol, Lanzhou 730000, Peoples R China.;Univ Bergen, Dept Clin Dent, Fac Med, N-5009 Bergen, Norway. (author)

Endochondral Ossification Induced by Cell Transplantation of Endothelial Cells and Bone Marrow Stromal Cells with Copolymer Scaffold Using a Rat Calvarial Defect Model

Article/chapterEnglish2021

Publisher, publication year, extent ...

2021-05-09
MDPI,2021
printrdacarrier

Numbers

LIBRIS-ID:oai:DiVA.org:kth-296195
https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-296195URI
https://doi.org/10.3390/polym13091521DOI

Supplementary language notes

Language:English
Summary in:English

Part of subdatabase

SwePubSwePub

Classification

Subject category:ref swepub-contenttype
Subject category:art swepub-publicationtype

Notes

QC 20210609
It has been recently reported that, in a rat calvarial defect model, adding endothelial cells (ECs) to a culture of bone marrow stromal cells (BMSCs) significantly enhanced bone formation. The aim of this study is to further investigate the ossification process of newly formed osteoid and host response to the poly(L-lactide-co-1,5-dioxepan-2-one) [poly(LLA-co-DXO)] scaffolds based on previous research. Several different histological methods and a PCR Array were applied to evaluate newly formed osteoid after 8 weeks after implantation. Histological results showed osteoid formed in rat calvarial defects and endochondral ossification-related genes, such as dentin matrix acidic phosphoprotein 1 (Dmp1) and collagen type II, and alpha 1 (Col2a1) exhibited greater expression in the CO (implantation with BMSC/EC/Scaffold constructs) than the BMSC group (implantation with BMSC/Scaffold constructs) as demonstrated by PCR Array. It was important to notice that cartilage-like tissue formed in the pores of the copolymer scaffolds. In addition, multinucleated giant cells (MNGCs) were observed surrounding the scaffold fragments. It was concluded that the mechanism of ossification might be an endochondral ossification process when the copolymer scaffolds loaded with co-cultured ECs/BMSCs were implanted into rat calvarial defects. MNGCs were induced by the poly(LLA-co-DXO) scaffolds after implantation, and more specific in vivo studies are needed to gain a better understanding of host response to copolymer scaffolds.

Subject headings and genre

MEDICIN OCH HÄLSOVETENSKAP Medicinsk bioteknologi Biomaterialvetenskap hsv//swe
MEDICAL AND HEALTH SCIENCES Medical Biotechnology Biomaterials Science hsv//eng
bone marrow stromal cells
endothelial cells
copolymer scaffold
endochondral ossification
host response

Added entries (persons, corporate bodies, meetings, titles ...)

Jiang, XiaofengLanzhou Univ, Sch Stomatol, Lanzhou 730000, Peoples R China. (author)
Si, QingzongLanzhou Univ, Sch Stomatol, Lanzhou 730000, Peoples R China. (author)
Finne Wistrand, Anna,1976-KTH,Polymerteknologi(Swepub:kth)u17gwfv7 (author)
Liu, BinLanzhou Univ, Sch Stomatol, Lanzhou 730000, Peoples R China. (author)
Xue, YingUniv Bergen, Dept Clin Dent, Fac Med, N-5009 Bergen, Norway. (author)
Mustafa, KamalUniv Bergen, Dept Clin Dent, Fac Med, N-5009 Bergen, Norway. (author)
Lanzhou Univ, Sch Stomatol, Lanzhou 730000, Peoples R China.;Univ Bergen, Dept Clin Dent, Fac Med, N-5009 Bergen, Norway.Lanzhou Univ, Sch Stomatol, Lanzhou 730000, Peoples R China. (creator_code:org_t)

Related titles

In:Polymers: MDPI13:92073-4360

Internet link

Find in a library

Polymers (Search for host publication in LIBRIS)

To the university's database

1 of 1
Previous record
Next record
To hitlist

Find more in SwePub

By the author/editor: Xing, Zhe; Jiang, Xiaofeng; Si, Qingzong; Finne Wistrand, ...; Liu, Bin; Xue, Ying; show more...; Mustafa, Kamal; show less...

About the subject

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Medical Biotechn ...; and Biomaterials Sci ...

Articles in the publication: Polymers

By the university: Royal Institute of Technology

Search outside SwePub

Extend your search to:: Google; Google Book Search; Google Scholar

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

LIBRIS.kb.se