Lipases for use in industrial biocatalysis : Specificity of selected structural groups of lipases

• Lipases for biocatalysis The substrate specificity of a selected group of lipases was investigated. The enzymes selected were from four structural groups. Group 1: lipases having wide alcohol binding cleft but a narrow acyl binding cleft (Rhizomucor miehei lipase. Thermomyces lanuginosus lipase. Fusarium oxysporum lipase); Group 2: lipases which exhibit strong restriction on the acid part having a narrow tunnel to accommodate the acyl group but wider alcohol binding site (Candida antarctica A, Candida rugosa lipase); Group 3: lipases having wide acyl binding cleft but narrow alcohol binding cleft (C. antarctica lipase B, Ustilago maydis lipase), and Group 4: having wider alcohol and wider acyl binding clefts (Fusarium solani pisi cutinase, Humicola insolens cutinase). Owing to the wide substrate specificity and higher expression levels in recombinant host, these lipases have tremendous importance for hydrolysis and synthesis reactions. Various substrates with substitutions on the alcohol and/or the acid part of the ester molecule were selected. The experimental results support the classification of lipases on the basis of their binding sites. For substrates with heavy alcohol side, C. Antarctica lipase A and R. miehei lipase type enzymes gave the highest extent of hydrolysis, while for acid heavy substrates the highest conversions were shown by C. antarctica lipase B. It is noteworthy that the acid heavy substrates which had aromatic side chains were hydrolyzed only by C. antarctica lipase B type of enzymes. Lipases were found to be more active on the alcohol-substituted substrates than acid-substituted substrates. (C) 2010 Elsevier B.V. All rights reserved.

Ämnesord

NATURVETENSKAP  -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)

NATURAL SCIENCES  -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)

NATURVETENSKAP  -- Kemi -- Fysikalisk kemi (hsv//swe)

NATURAL SCIENCES  -- Chemical Sciences -- Physical Chemistry (hsv//eng)

Nyckelord

Lipase

Biocatalysis

Substrate specificity

Enantio selectivity

Biochemistry

Biokemi

Molecular biology

Molekylärbiologi

Physical chemistry

Fysikalisk kemi

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