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Production of β-carotene by recombinant Escherichia coli with engineered whole mevalonate pathway in batch and fed-batch cultures

Kim, Jung Hun (author)
Department of Chemical & Biological Engineering and ERI, Gyeongsang National University, Jinju, 660-701, Korea
Kim, Seon Won (author)
Division of Applied Life Science (BK21), EB-NCRC and PMBBRC, Gyeongsang National University, Jinju, 660-701, Korea
Nguyen, Do Quynh Anh (author)
Department of Chemical & Biological Engineering and ERI, Gyeongsang National University, Jinju, 660-701, Korea
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Li, He, D.Sc, 1985- (author)
Department of Chemical & Biological Engineering and ERI, Gyeongsang National University, Jinju, 660-701, Korea
Kim, Sung Bae (author)
Department of Chemical & Biological Engineering and ERI, Gyeongsang National University, Jinju, 660-701, Korea
Seo, Yang Gon (author)
Department of Chemical & Biological Engineering and ERI, Gyeongsang National University, Jinju, 660-701, Korea
Yang, Jae Kyung (author)
Division of Environmental Forest Science and Institute of Agriculture & Life Science, Gyeongsang National University, Jinju, 660-701, Korea
Chung, In Young (author)
Department of Electronic and Communications Engineering, Kwangwoon University, Seoul, 139-701, Korea
Kim, Dae Hwan (author)
School of Electrical Engineering, Kookmin University, Seoul, 136-702, Korea
Kim, Chang Joon (author)
Department of Chemical & Biological Engineering and ERI, Gyeongsang National University, Jinju, 660-701, Korea
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 (creator_code:org_t)
2009-11-05
2009
English.
In: Biotechnology and Bioprocess Engineering. - : Springer Science and Business Media LLC. - 1226-8372 .- 1976-3816. ; 14:5, s. 559-564
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https://urn.kb.se/re...
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  • Journal article (peer-reviewed)
Abstract Subject headings
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  • Recombinant Escherichia coli engineered to contain the whole mevalonate pathway and foreign genes for β-carotene biosynthesis, was utilized for production of β-carotene in bioreactor cultures. Optimum culture conditions were established in batch and pH-stat fed-batch cultures to determine the optimal feeding strategy thereby improving production yield. The specific growth rate and volumetric productivity in batch cultures at 37°C were 1.7-fold and 2-fold higher, respectively, than those at 28°C. Glycerol was superior to glucose as a carbon source. Maximum β-carotene production (titer of 663 mg/L and overall volumetric productivity of 24.6 mg/L × h) resulted from the simultaneous addition of 500 g/L glycerol and 50 g/L yeast extract in pH-stat fed-batch culture.

Subject headings

TEKNIK OCH TEKNOLOGIER  -- Industriell bioteknik -- Bioprocessteknik (hsv//swe)
ENGINEERING AND TECHNOLOGY  -- Industrial Biotechnology -- Bioprocess Technology (hsv//eng)

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