Production of β-carotene by recombinant Escherichia coli with engineered whole mevalonate pathway in batch and fed-batch cultures

Kim, Jung HunDepartment of Chemical & Biological Engineering and ERI, Gyeongsang National University, Jinju, 660-701, Korea (author)

QC 20210824
Recombinant Escherichia coli engineered to contain the whole mevalonate pathway and foreign genes for β-carotene biosynthesis, was utilized for production of β-carotene in bioreactor cultures. Optimum culture conditions were established in batch and pH-stat fed-batch cultures to determine the optimal feeding strategy thereby improving production yield. The specific growth rate and volumetric productivity in batch cultures at 37°C were 1.7-fold and 2-fold higher, respectively, than those at 28°C. Glycerol was superior to glucose as a carbon source. Maximum β-carotene production (titer of 663 mg/L and overall volumetric productivity of 24.6 mg/L × h) resulted from the simultaneous addition of 500 g/L glycerol and 50 g/L yeast extract in pH-stat fed-batch culture.

Kim, Seon WonDivision of Applied Life Science (BK21), EB-NCRC and PMBBRC, Gyeongsang National University, Jinju, 660-701, Korea (author)
Nguyen, Do Quynh AnhDepartment of Chemical & Biological Engineering and ERI, Gyeongsang National University, Jinju, 660-701, Korea (author)
Li, He,D.Sc,1985-Department of Chemical & Biological Engineering and ERI, Gyeongsang National University, Jinju, 660-701, Korea(Swepub:kth)u192ayff (author)
Kim, Sung BaeDepartment of Chemical & Biological Engineering and ERI, Gyeongsang National University, Jinju, 660-701, Korea (author)
Seo, Yang GonDepartment of Chemical & Biological Engineering and ERI, Gyeongsang National University, Jinju, 660-701, Korea (author)
Yang, Jae KyungDivision of Environmental Forest Science and Institute of Agriculture & Life Science, Gyeongsang National University, Jinju, 660-701, Korea (author)
Chung, In YoungDepartment of Electronic and Communications Engineering, Kwangwoon University, Seoul, 139-701, Korea (author)
Kim, Dae HwanSchool of Electrical Engineering, Kookmin University, Seoul, 136-702, Korea (author)
Kim, Chang JoonDepartment of Chemical & Biological Engineering and ERI, Gyeongsang National University, Jinju, 660-701, Korea (author)
Department of Chemical & Biological Engineering and ERI, Gyeongsang National University, Jinju, 660-701, KoreaDivision of Applied Life Science (BK21), EB-NCRC and PMBBRC, Gyeongsang National University, Jinju, 660-701, Korea (creator_code:org_t)

In:Biotechnology and Bioprocess Engineering: Springer Science and Business Media LLC14:5, s. 559-5641226-83721976-3816

By the author/editor: Kim, Jung Hun; Kim, Seon Won; Nguyen, Do Quynh ...; Li, He, D.Sc, 19 ...; Kim, Sung Bae; Seo, Yang Gon; show more...; Yang, Jae Kyung; Chung, In Young; Kim, Dae Hwan; Kim, Chang Joon; show less...

About the subject

ENGINEERING AND TECHNOLOGY: ENGINEERING AND ...; and Industrial Biote ...; and Bioprocess Techn ...

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