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Anti-wrinkle activi...
Anti-wrinkle activity of beta-carotene extracted & purified from recombinant Escherichia coli
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- Jo, Ji Song (author)
- Department of Chemical & Biological Engineering and ERI, GyeongSang National University
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- Ku, Bo Mi (author)
- Department of Anatomy & Neurobiology, GyeongSang National University
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- Kang, Sang Soo (author)
- Department of Anatomy & Neurobiology, GyeongSang National University
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- Lee, Jae Ran (author)
- Department of Chemical & Biological Engineering and ERI, GyeongSang National University
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- Kim, You Geun (author)
- KB Cosmetics
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- Li, He, 1985- (author)
- Department of Chemical & Biological Engineering and ERI, GyeongSang National University
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- Kim, Sung-Bae (author)
- (Department of Chemical & Biological Engineering and ERI, GyeongSang National University
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- Kim, Seon-Won (author)
- Division of Applied Life Science, GyeongSang National University
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- Kim, Chang-Joon (author)
- Department of Chemical & Biological Engineering and ERI, GyeongSang National University
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- Chung, In-Young (author)
- Department of Electronics and Communications, Kwangwoon University
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(creator_code:org_t)
- 2008
- 2008
- Korean.
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In: KSBB Journal. - 1225-7117 .- 2288-8268. ; 23:6, s. 513-518
- Related links:
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https://www.koreasci...
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https://urn.kb.se/re...
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Abstract
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- This paper described the extraction/purification of β-carotene from recombinant E.coli and evaluation of anti-wrinkle activity of purified β-carotene. No significant differences in extraction yields were observed when hexane or isobutyl acetate was used. However, extraction from wet-cell cake resulted in 2-fold higher amount of β-carotene than that from dry cells. Disruption of 5 g-wet cells by ultrasonic homogenizer, acetone dehydration, extraction with isobutyl acetate resulted in 36 mg of β-carotene corresponding to 61.2% of recovery. The formation and separation of ββ-carotene crystal improved the purity. 633 mg of β-carotene crystal with 93% purity was obtained from 223 g/L of wet-cell cake harvested from 2.5-L fed-batch culture broth. The cultures of normal human primary fibroblast were performed to investigate the effect of β-carotene on cytotoxicity as MTT assay and anti-wrinkle activity as collagen synthesis assays. 1.7μM of β-carotene was found to be optimal concentration at which 1.4-fold higher amount of collagen was synthesized than that in absence of β-carotene. This indicates that highly purified β-carotene can be obtained from recombinant E.coli by applying simple method with less toxic solvent and can be used in functional cosmetics as anti-wrinkle agent.
- 재조합 대장균으로부터 베타-카로틴을 추출/정제하고 정제된 베타-카로틴의 주름개선 성능을 평가하였다. 탈수 용매, 추출 용매에 따른 추출량의 큰 변화는 발견되지 않았으나, wet-cell cake으로부터 추출한 경우가 dry cell로부터 추출한 경우에 비하여 2배 많은 양의 베타-카로틴이 추출되었다. 5 g의 wet-cell cake을 파쇄하고, 아세톤으로 탈수한 후, isobutyl acetate로 추출한 결과 36 mg의 베타-카로틴이 추출되었는데, 이는 61.2% 회수율에 해당한다. 베타-카로틴의 결정을 생성시키고 이를 분리한 결과 순도가 증가하였다. 재조합 대장균 배양액 2.5 L로부터 얻은 wet-cell cake 223 g의 탈수, 세포 파쇄 후 세포 잔사물로부터 베타-카로틴 추출, 농축, 결정화 등을 통하여 최종적으로 순도 93%인 베타-카로틴 결정 633 mg을 얻었다. 사람섬유아세포 배양을 통하여 정제된 베타-카로틴의 세포 독성 및 콜라겐 합성에 미치는 영향을 조사하였다. 1.7μM1.7μM의 베타-카로틴을 첨가 시 세포에 독성을 거의 나타내지 않았으며 콜라겐 합성양은 첨가하지 않은 경우에 비하여 1.4배 증가하였다. 본 결과는 저 유독성 용매를 사용하고 기존보다 간단한 방법을 사용하여 재조합 대장균으로부터 고순도의 베타-카로틴을 정제할 수 있고 이를 기능성 화장품의 주름개선제로 사용될 수 있음을 시사한다.
Subject headings
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
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- ref (subject category)
- art (subject category)
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- By the author/editor
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Jo, Ji Song
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Ku, Bo Mi
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Kang, Sang Soo
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Lee, Jae Ran
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Kim, You Geun
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Li, He, 1985-
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show more...
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Kim, Sung-Bae
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Kim, Seon-Won
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Kim, Chang-Joon
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Chung, In-Young
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- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Biochemistry and ...
- Articles in the publication
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KSBB Journal
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Royal Institute of Technology