High-content nucleus based 3D image cytometery of whole multicellular tumour spheroids

QC 20210927
We have previously presented an ultrasonic standing wave (USW) based multicellular tumor spheroid (MCTS) culture platform where 100 MCST can be formed, processed and imaged while being retained in micro-wells [1]. This platform was used to show MCTS characterization based on on-chip microscopy and off-chip flow cytometry [2]. Here, we present a high-content 3D image cytometry method of whole MCTS based on DNA staining where spatial and functional information can be assessed.

MEDICIN OCH HÄLSOVETENSKAP Medicinska och farmaceutiska grundvetenskaper Cell- och molekylärbiologi hsv//swe
MEDICAL AND HEALTH SCIENCES Basic Medicine Cell and Molecular Biology hsv//eng
Confocal imaging
High-content cytometry
Image analysis
Multicellular tumor spheroids
3-D image
Cytometry
Functional information
High-content
Micro wells
Ultrasonic standing waves
Tumors

Carannante, ValentinaKarolinska Institutet, Sweden (author)
Önfelt, BjörnKTH,Biomedicinsk fysik och röntgenfysik,Karolinska Institutet, Sweden(Swepub:kth)u1tquq43 (author)
Wiklund, MartinKTH,Biomedicinsk fysik och röntgenfysik(Swepub:kth)u1rillco (author)
KTHBiomedicinsk fysik och röntgenfysik (creator_code:org_t)

In:22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018: Chemical and Biological Microsystems Society, s. 1433-1434

By the author/editor: Olofsson, Karl; Carannante, Vale ...; Önfelt, Björn; Wiklund, Martin

About the subject

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Basic Medicine; and Cell and Molecul ...

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