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A Combination of N ...
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Jalkanen, PinjaUniv Turku, Inst Biomed, Infect & Immunol Unit, Turku, Finland.
(author)
A Combination of N and S Antigens With IgA and IgG Measurement Strengthens the Accuracy of SARS-CoV-2 Serodiagnostics
- Article/chapterEnglish2021
Publisher, publication year, extent ...
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2021-04-27
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Oxford University Press,2021
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printrdacarrier
Numbers
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LIBRIS-ID:oai:DiVA.org:kth-302679
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https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-302679URI
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https://doi.org/10.1093/infdis/jiab222DOI
Supplementary language notes
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Language:English
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Summary in:English
Part of subdatabase
Classification
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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QC 20210930
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Background. Primary diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection is based on detection of virus RNA in nasopharyngeal swab samples. In addition, analysis of humoral immunity against SARS-CoV-2 has an important role in viral diagnostics and seroprevalence estimates. Methods. We developed and optimized an enzyme immunoassays (EIA) using SARS-CoV-2 nucleoprotein (N), Si and receptor binding domain (RBD) of the viral spike protein, and N proteins from SARS, Middle East respiratory syndrome (MERS), and 4 low-pathogenic human CoVs. Neutralizing antibody activity was compared with SARS-CoV-2 IgG, IgA, and IgM EIA results. Results. The sensitivity of EIA for detecting immune response in COVID-19 patients (n = 101) was 77% in the acute phase and 100% in the convalescent phase of SARS-CoV-2 infection when N and RBD were used as antigens in IgG and IgA specific EIAs. SARS-CoV-2 infection significantly increased humoral immune responses against the 229E and NL63 N proteins. Si and RBD-based EIA results had a strong correlation with microneutralization test results. Conclusions. The data indicate a combination of SARS-CoV-2 Si or RBD and N proteins and analysis of IgG and IgA immunoglobulin classes in sera provide an excellent basis for specific and sensitive serological diagnostics of COVID-19.
Subject headings and genre
Added entries (persons, corporate bodies, meetings, titles ...)
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Pasternack, ArjaUniv Helsinki, Dept Physiol, Biomed, Helsinki, Finland.
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Maljanen, SariUniv Turku, Inst Biomed, Infect & Immunol Unit, Turku, Finland.
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Melen, KristerUniv Turku, Inst Biomed, Infect & Immunol Unit, Turku, Finland.;Finnish Inst Hlth & Welf, Helsinki, Finland.
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Kolehmainen, PekkaUniv Turku, Inst Biomed, Infect & Immunol Unit, Turku, Finland.
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Huttunen, MoonaUniv Turku, Inst Biomed, Infect & Immunol Unit, Turku, Finland.
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Lundberg, RickardUniv Turku, Inst Biomed, Infect & Immunol Unit, Turku, Finland.
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Tripathi, LavUniv Turku, Inst Biomed, Infect & Immunol Unit, Turku, Finland.
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Khan, HiraUniv Turku, Inst Biomed, Infect & Immunol Unit, Turku, Finland.
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Ritvos, Mikael A.KTH,Skolan för kemi, bioteknologi och hälsa (CBH),Univ Helsinki, Dept Physiol, Biomed, Helsinki, Finland. Nordic SARS Response AB, Stockholm, Sweden.(Swepub:kth)u188rdhl
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Naves, RaunoUniv Helsinki, Dept Physiol, Biomed, Helsinki, Finland.
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Haveri, AnuFinnish Inst Hlth & Welf, Helsinki, Finland.
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Osterlund, PamelaFinnish Inst Hlth & Welf, Helsinki, Finland.
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Kuivanen, SuviUniv Helsinki, Dept Virol, Helsinki, Finland.
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Jaaskelainen, Anne J.Univ Helsinki, HUS Diagnost Ctr, HUSLAB, Clin Microbiol, Helsinki, Finland.;Helsinki Univ Hosp, Helsinki, Finland.
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Kurkela, SatuUniv Helsinki, HUS Diagnost Ctr, HUSLAB, Clin Microbiol, Helsinki, Finland.;Helsinki Univ Hosp, Helsinki, Finland.
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Lappalainen, MaijaUniv Helsinki, HUS Diagnost Ctr, HUSLAB, Clin Microbiol, Helsinki, Finland.;Helsinki Univ Hosp, Helsinki, Finland.
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Rantasarkka, KaisaTurku Univ Hosp, Clin Microbiol, Turku, Finland.
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Vuorinen, TyttiUniv Turku, Inst Biomed, Infect & Immunol Unit, Turku, Finland.;Turku Univ Hosp, Clin Microbiol, Turku, Finland.
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Hytonen, JukkaUniv Turku, Inst Biomed, Infect & Immunol Unit, Turku, Finland.;Turku Univ Hosp, Clin Microbiol, Turku, Finland.
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Waris, MattiUniv Turku, Inst Biomed, Infect & Immunol Unit, Turku, Finland.;Turku Univ Hosp, Clin Microbiol, Turku, Finland.
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Tauriainen, SiskoUniv Turku, Inst Biomed, Infect & Immunol Unit, Turku, Finland.
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Ritvos, OlliUniv Helsinki, Dept Physiol, Biomed, Helsinki, Finland.
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Kakkola, LauraUniv Turku, Inst Biomed, Infect & Immunol Unit, Turku, Finland.
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Julkunen, IlkkaUniv Turku, Inst Biomed, Infect & Immunol Unit, Turku, Finland.;Turku Univ Hosp, Clin Microbiol, Turku, Finland.
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Univ Turku, Inst Biomed, Infect & Immunol Unit, Turku, Finland.Univ Helsinki, Dept Physiol, Biomed, Helsinki, Finland.
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In:Journal of Infectious Diseases: Oxford University Press224:2, s. 218-2280022-18991537-6613
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Jalkanen, Pinja
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Pasternack, Arja
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Maljanen, Sari
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Melen, Krister
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Kolehmainen, Pek ...
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Huttunen, Moona
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Lundberg, Rickar ...
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Tripathi, Lav
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Khan, Hira
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Ritvos, Mikael A ...
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Naves, Rauno
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Haveri, Anu
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Osterlund, Pamel ...
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Kuivanen, Suvi
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Jaaskelainen, An ...
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Kurkela, Satu
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Lappalainen, Mai ...
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Rantasarkka, Kai ...
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Vuorinen, Tytti
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Hytonen, Jukka
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Waris, Matti
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Tauriainen, Sisk ...
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Ritvos, Olli
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Kakkola, Laura
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Julkunen, Ilkka
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