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  • Jalkanen, PinjaUniv Turku, Inst Biomed, Infect & Immunol Unit, Turku, Finland. (author)

A Combination of N and S Antigens With IgA and IgG Measurement Strengthens the Accuracy of SARS-CoV-2 Serodiagnostics

  • Article/chapterEnglish2021

Publisher, publication year, extent ...

  • 2021-04-27
  • Oxford University Press,2021
  • printrdacarrier

Numbers

  • LIBRIS-ID:oai:DiVA.org:kth-302679
  • https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-302679URI
  • https://doi.org/10.1093/infdis/jiab222DOI

Supplementary language notes

  • Language:English
  • Summary in:English

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  • Subject category:ref swepub-contenttype
  • Subject category:art swepub-publicationtype

Notes

  • QC 20210930
  • Background. Primary diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection is based on detection of virus RNA in nasopharyngeal swab samples. In addition, analysis of humoral immunity against SARS-CoV-2 has an important role in viral diagnostics and seroprevalence estimates. Methods. We developed and optimized an enzyme immunoassays (EIA) using SARS-CoV-2 nucleoprotein (N), Si and receptor binding domain (RBD) of the viral spike protein, and N proteins from SARS, Middle East respiratory syndrome (MERS), and 4 low-pathogenic human CoVs. Neutralizing antibody activity was compared with SARS-CoV-2 IgG, IgA, and IgM EIA results. Results. The sensitivity of EIA for detecting immune response in COVID-19 patients (n = 101) was 77% in the acute phase and 100% in the convalescent phase of SARS-CoV-2 infection when N and RBD were used as antigens in IgG and IgA specific EIAs. SARS-CoV-2 infection significantly increased humoral immune responses against the 229E and NL63 N proteins. Si and RBD-based EIA results had a strong correlation with microneutralization test results. Conclusions. The data indicate a combination of SARS-CoV-2 Si or RBD and N proteins and analysis of IgG and IgA immunoglobulin classes in sera provide an excellent basis for specific and sensitive serological diagnostics of COVID-19.

Subject headings and genre

Added entries (persons, corporate bodies, meetings, titles ...)

  • Pasternack, ArjaUniv Helsinki, Dept Physiol, Biomed, Helsinki, Finland. (author)
  • Maljanen, SariUniv Turku, Inst Biomed, Infect & Immunol Unit, Turku, Finland. (author)
  • Melen, KristerUniv Turku, Inst Biomed, Infect & Immunol Unit, Turku, Finland.;Finnish Inst Hlth & Welf, Helsinki, Finland. (author)
  • Kolehmainen, PekkaUniv Turku, Inst Biomed, Infect & Immunol Unit, Turku, Finland. (author)
  • Huttunen, MoonaUniv Turku, Inst Biomed, Infect & Immunol Unit, Turku, Finland. (author)
  • Lundberg, RickardUniv Turku, Inst Biomed, Infect & Immunol Unit, Turku, Finland. (author)
  • Tripathi, LavUniv Turku, Inst Biomed, Infect & Immunol Unit, Turku, Finland. (author)
  • Khan, HiraUniv Turku, Inst Biomed, Infect & Immunol Unit, Turku, Finland. (author)
  • Ritvos, Mikael A.KTH,Skolan för kemi, bioteknologi och hälsa (CBH),Univ Helsinki, Dept Physiol, Biomed, Helsinki, Finland. Nordic SARS Response AB, Stockholm, Sweden.(Swepub:kth)u188rdhl (author)
  • Naves, RaunoUniv Helsinki, Dept Physiol, Biomed, Helsinki, Finland. (author)
  • Haveri, AnuFinnish Inst Hlth & Welf, Helsinki, Finland. (author)
  • Osterlund, PamelaFinnish Inst Hlth & Welf, Helsinki, Finland. (author)
  • Kuivanen, SuviUniv Helsinki, Dept Virol, Helsinki, Finland. (author)
  • Jaaskelainen, Anne J.Univ Helsinki, HUS Diagnost Ctr, HUSLAB, Clin Microbiol, Helsinki, Finland.;Helsinki Univ Hosp, Helsinki, Finland. (author)
  • Kurkela, SatuUniv Helsinki, HUS Diagnost Ctr, HUSLAB, Clin Microbiol, Helsinki, Finland.;Helsinki Univ Hosp, Helsinki, Finland. (author)
  • Lappalainen, MaijaUniv Helsinki, HUS Diagnost Ctr, HUSLAB, Clin Microbiol, Helsinki, Finland.;Helsinki Univ Hosp, Helsinki, Finland. (author)
  • Rantasarkka, KaisaTurku Univ Hosp, Clin Microbiol, Turku, Finland. (author)
  • Vuorinen, TyttiUniv Turku, Inst Biomed, Infect & Immunol Unit, Turku, Finland.;Turku Univ Hosp, Clin Microbiol, Turku, Finland. (author)
  • Hytonen, JukkaUniv Turku, Inst Biomed, Infect & Immunol Unit, Turku, Finland.;Turku Univ Hosp, Clin Microbiol, Turku, Finland. (author)
  • Waris, MattiUniv Turku, Inst Biomed, Infect & Immunol Unit, Turku, Finland.;Turku Univ Hosp, Clin Microbiol, Turku, Finland. (author)
  • Tauriainen, SiskoUniv Turku, Inst Biomed, Infect & Immunol Unit, Turku, Finland. (author)
  • Ritvos, OlliUniv Helsinki, Dept Physiol, Biomed, Helsinki, Finland. (author)
  • Kakkola, LauraUniv Turku, Inst Biomed, Infect & Immunol Unit, Turku, Finland. (author)
  • Julkunen, IlkkaUniv Turku, Inst Biomed, Infect & Immunol Unit, Turku, Finland.;Turku Univ Hosp, Clin Microbiol, Turku, Finland. (author)
  • Univ Turku, Inst Biomed, Infect & Immunol Unit, Turku, Finland.Univ Helsinki, Dept Physiol, Biomed, Helsinki, Finland. (creator_code:org_t)

Related titles

  • In:Journal of Infectious Diseases: Oxford University Press224:2, s. 218-2280022-18991537-6613

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