Tem grid preparation with minimal user interaction

• Transmission electron microscopy (TEM) allows to directly visualize and characterize small biological particles, such as viruses, making it an indispensable tool for development of vaccines and pharmaceuticals. However, the manual TEM grid preparation protocol strongly depends on the skill of the operator and affects the preparation consistency. Here, we present a capillary-driven microfluidic device for negative stain TEM (nsTEM) grid preparation with minimal user interaction. The presented device could make TEM grid preparations more consistent and provide non-specialists access to biological TEM investigations.

Ämnesord

NATURVETENSKAP  -- Kemi -- Fysikalisk kemi (hsv//swe)

NATURAL SCIENCES  -- Chemical Sciences -- Physical Chemistry (hsv//eng)

Nyckelord

Capillary-driven

Electron microscopy

Sample preparation

TEM

Virus

Viruses

Biological particles

Indispensable tools

Micro-fluidic devices

TEM grids

User interaction

High resolution transmission electron microscopy

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