An automated microfluidic diagnostics pipeline for infectious disease detection in low resource settings

• While diagnostics are critical for accurate and timely diagnosis, gold-standard diagnostic tests are commonly high- performance laboratory-based tests that require multi-step protocols for complex sample processing and highly trained personnel, both scarce in low-resource settings. Here, we address the need for an easy-to-use, rapid and reliable diagnostic testing pipeline by presenting a solution combining open-source modular automation and automation compatible microfluidics, easily adaptable to a pipeline for infectious diseases diagnosis. We demonstrate an automation compatible microfluidics pipeline for Neisseria meningitidis diagnosis by on-chip nucleic acids isolation and isothermal amplification, as well as pathogen detection on a paper-based microarray. 

Ämnesord

MEDICIN OCH HÄLSOVETENSKAP  -- Klinisk medicin -- Infektionsmedicin (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Clinical Medicine -- Infectious Medicine (hsv//eng)

Nyckelord

Automation

Diagnostics

Global health

Infectious diseases

Microfluidics

Modular robotics

Diseases

Nucleic acids

Pipelines

Diagnostic testing

Diagnostic tests

Infectious disease

Isothermal amplifications

Low-resource settings

Multi step protocols

Neisseria meningitidis

Pathogen detection

Diagnosis

Publikations- och innehållstyp

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