Sökning: WFRF:(Iyengar Sharath)
> (2021) >
High resolution and...
High resolution and rapid separation of bacteria from blood using elasto‐inertial microfluidics
-
- Iyengar, Sharath Narayana (författare)
- KTH,Nanobioteknologi,Proteinvetenskap,Science for Life Laboratory, SciLifeLab,AIMES—Center for the Advancement of Integrated Medical and Engineering Sciences at Karolinska Institutet and KTH Royal Institute of Technology
-
- Kumar, Tharagan (författare)
- KTH,Nanobioteknologi,AIMES—Center for the Advancement of Integrated Medical and Engineering Sciences at Karolinska Institutet and KTH Royal Institute of Technology
-
- Mårtensson, Gustaf (författare)
- KTH,Nanobioteknologi
-
visa fler...
-
- Russom, Aman, Prof. 1976- (författare)
- KTH,Science for Life Laboratory, SciLifeLab,Nanobioteknologi,AIMES—Center for the Advancement of Integrated Medical and Engineering Sciences at Karolinska Institutet and KTH Royal Institute of Technology
-
visa färre...
-
(creator_code:org_t)
- 2021-09-23
- 2021
- Engelska.
-
Ingår i: Electrophoresis. - : Wiley. - 0173-0835 .- 1522-2683. ; 42:23, s. 2538-2551
- Relaterad länk:
-
https://doi.org/10.1...
-
visa fler...
-
https://kth.diva-por... (primary) (Raw object)
-
https://onlinelibrar...
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
http://kipublication...
-
visa färre...
Abstract
Ämnesord
Stäng
- Improved sample preparation has the potential to address unmet needs for fast turnaroundsepsis tests. In this work, we report elasto-inertial based rapid bacteria separation from diluted blood at high separation efficiency. In viscoelastic flows, we demonstrate novel findings where blood cells prepositioned at the outer wall entering a spiral device remain fullyfocused throughout the channel length while smaller bacteria migrate to the opposite wall.Initially, using microparticles, we show that particles above a certain size cut-off remainfully focused at the outer wall while smaller particles differentially migrate toward the inner wall. We demonstrate particle separation at 1 μm resolution at a total throughput of1 mL/min. For blood-based experiments, a minimum of 1:2 dilution was necessary to fullyfocus blood cells at the outer wall. Finally, Escherichia coli spiked in diluted blood were continuously separated at a total flow rate of 1 mL/min, with efficiencies between 82 and 90%depending on the blood dilution. Using a single spiral, it takes 40 min to process 1 mLof blood at a separation efficiency of 82%. The label-free, passive, and rapid bacteria isolation method has a great potential for speeding up downstream phenotypic and genotypicanalysis.
Ämnesord
- NATURVETENSKAP -- Kemi -- Analytisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Analytical Chemistry (hsv//eng)
Nyckelord
- Clinical Biochemistry
- Biochemistry
- Analytical Chemistry
Publikations- och innehållstyp
- vet (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas