Coming of Age: Cryo-Electron Tomography as a Versatile Tool to Generate High-Resolution Structures at Cellular/Biological Interfaces

• Over the last few years, cryo electron microscopy has become the most important methodin structural biology. While 80% of deposited maps are from single particle analysis, electrontomography has grown to become the second most important method. In particular sub-tomogramaveraging has matured as a method, delivering structures between 2 and 5 Å from complexes in cellsas well as in vitro complexes. While this resolution range is not standard, novel developments pointtoward a promising future. Here, we provide a guide for the workflow from sample to structure togain insight into this emerging field.

Nyckelord

Inorganic Chemistry

Organic Chemistry

Physical and Theoretical Chemistry

Computer Science Applications

Spectroscopy

Molecular Biology

General Medicine

Catalysis

Bioteknologi

Biotechnology

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