3D Particle-Free Printing of Biocompatible Conductive Hydrogel Platforms for Neuron Growth and Electrophysiological Recording

• Electrically conductive 3D periodic microscaffolds are fabricated using a particle-free direct ink writing approach for use as neuronal growth and electrophysiological recording platforms. A poly (2-hydroxyethyl methacrylate)/pyrrole ink, followed by chemical in situ polymerization of pyrrole, enables hydrogel printing through nozzles as small as 1 µm. These conductive hydrogels can pattern complex 2D and 3D structures and have good biocompatibility with test cell cultures (≈94.5% viability after 7 days). Hydrogel arrays promote extensive neurite outgrowth of cultured Aplysia californica pedal ganglion neurons. This platform allows extracellular electrophysiological recording of steady-state and stimulated electrical neuronal activities. In summation, this 3D conductive ink printing process enables the preparation of biocompatible and micron-sized structures to create customized in vitro electrophysiological recording platforms.

Ämnesord

TEKNIK OCH TEKNOLOGIER  -- Medicinteknik -- Medicinsk material- och protesteknik (hsv//swe)

ENGINEERING AND TECHNOLOGY  -- Medical Engineering -- Medical Materials (hsv//eng)

TEKNIK OCH TEKNOLOGIER  -- Materialteknik -- Textil-, gummi- och polymermaterial (hsv//swe)

ENGINEERING AND TECHNOLOGY  -- Materials Engineering -- Textile, Rubber and Polymeric Materials (hsv//eng)

Nyckelord

3D printing

Aplysia californica

conductive hydrogel

microfabricated neuron recording

Aromatic compounds

Biocompatibility

Cell culture

Electrophysiology

Neurons

PHEMA

Electrically conductive

Electrophysiological recordings

In-situ polymerization

Neurite outgrowth

Neuronal activities

Poly(2-hydroxyethyl methacrylate)

Printing process

3D printers

Publikations- och innehållstyp

ref (ämneskategori)

art (ämneskategori)