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A silicon-glass mic...
A silicon-glass microwell platform for high-resolution imaging and high-content screening with single cell resolution
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- Frisk, Thomas (författare)
- KTH,Cellens fysik
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- Khorshidi, Mohammad Ali (författare)
- KTH,Cellens fysik
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- Guldevall, Karolin (författare)
- KTH,Cellens fysik
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- Vanherberghen, Bruno (författare)
- KTH,Cellens fysik
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- Önfelt, Björn (författare)
- Karolinska Institutet,KTH,Cellens fysik
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(creator_code:org_t)
- 2011-04-05
- 2011
- Engelska.
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Ingår i: Biomedical microdevices (Print). - : Springer Science and Business Media LLC. - 1387-2176 .- 1572-8781. ; 13:4, s. 683-693
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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http://kipublication...
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Abstract
Ämnesord
Stäng
- We present a novel microwell array platform suited for various cell-imaging assays where single cell resolution is important. The platform consists of an exchangeable silicon-glass microchip for cell biological applications and a custom made holder that fits in conventional microscopes. The microchips presented here contain arrays of miniature wells, where the well sizes and layout have been designed for different applications, including single cell imaging, studies of cell-cell interactions or ultrasonic manipulation of cells. The device has been designed to be easy to use, to allow long-term assays (spanning several days) with read-outs based on high-resolution imaging or high-content screening. This study is focused on screening applications and an automatic cell counting protocol is described and evaluated. Finally, we have tested the device and automatic counting by studying the selective survival and clonal expansion of 721.221 B cells transfected to express HLA Cw6-GFP compared to untransfected 721.221 B cells when grown under antibiotic selection for 3 days. The device and automated analysis protocol make up the foundation for development of several novel cellular imaging assays.
Ämnesord
- TEKNIK OCH TEKNOLOGIER -- Industriell bioteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Industrial Biotechnology (hsv//eng)
Nyckelord
- single cell
- microwell
- automatic image analysis
- screening fluorerscence imaging
- clonal expansion
- Bioengineering
- Bioteknik
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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