Sökning: WFRF:(Cioci Gianluca)
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A Soluble Fucose-Sp...
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Houser, JosefMasaryk Univ, Cent European Inst Technol, Brno, Czech Republic.;Masaryk Univ, Fac Sci, Natl Ctr Biomol Res, CS-61137 Brno, Czech Republic.
(författare)
A Soluble Fucose-Specific Lectin from Aspergillus fumigatus Conidia - Structure, Specificity and Possible Role in Fungal Pathogenicity
- Artikel/kapitelEngelska2013
Förlag, utgivningsår, omfång ...
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2013-12-10
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Public Library of Science (PLoS),2013
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printrdacarrier
Nummerbeteckningar
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LIBRIS-ID:oai:DiVA.org:kth-305698
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https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-305698URI
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https://doi.org/10.1371/journal.pone.0083077DOI
Kompletterande språkuppgifter
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Språk:engelska
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Sammanfattning på:engelska
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Klassifikation
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Ämneskategori:ref swepub-contenttype
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Ämneskategori:art swepub-publicationtype
Anmärkningar
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QC 20211206
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Aspergillus fumigatus is an important allergen and opportunistic pathogen. Similarly to many other pathogens, it is able to produce lectins that may be involved in the host-pathogen interaction. We focused on the lectin AFL, which was prepared in recombinant form and characterized. Its binding properties were studied using hemagglutination and glycan array analysis. We determined the specificity of the lectin towards l-fucose and fucosylated oligosaccharides, including alpha 1-6 linked core-fucose, which is an important marker for cancerogenesis. Other biologically relevant saccharides such as sialic acid, d-mannose or d-galactose were not bound. Blood group epitopes of the ABH and Lewis systems were recognized, Le(Y) being the preferred ligand among others. To provide a correlation between the observed functional characteristics and structural basis, AFL was crystallized in a complex with methyl-alpha,L-selenofucoside and its structure was solved using the SAD method. Six binding sites, each with different compositions, were identified per monomer and significant differences from the homologous AAL lectin were found. Structure-derived peptides were utilized to prepare anti-AFL polyclonal antibodies, which suggested the presence of AFL on the Aspergillus' conidia, confirming its expression in vivo. Stimulation of human bronchial cells by AFL led to IL-8 production in a dose-dependent manner. AFL thus probably contributes to the inflammatory response observed upon the exposure of a patient to A. fumigatus. The combination of affinity to human epithelial epitopes, production by conidia and pro-inflammatory activity is remarkable and shows that AFL might be an important virulence factor involved in an early stage of A. fumigatus infection.
Ämnesord och genrebeteckningar
Biuppslag (personer, institutioner, konferenser, titlar ...)
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Komarek, JanMasaryk Univ, Fac Sci, Natl Ctr Biomol Res, CS-61137 Brno, Czech Republic.
(författare)
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Kostlanova, NikolaMasaryk Univ, Cent European Inst Technol, Brno, Czech Republic.;Masaryk Univ, Fac Sci, Natl Ctr Biomol Res, CS-61137 Brno, Czech Republic.
(författare)
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Cioci, GianlucaEuropean Synchrotron Radiat Facil, F-38043 Grenoble, France.
(författare)
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Varrot, AnnabelleUniv Grenoble, CERMAV CNRS, Grenoble, France.
(författare)
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Kerr, Sheena C.Univ Calif San Francisco, Dept Med, San Francisco, CA USA.;Univ Calif San Francisco, CVRI, San Francisco, CA 94143 USA.
(författare)
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Lahmann, MartinaUniv Bangor, Sch Chem, Bangor, Gwynedd, Wales.(Swepub:kth)u190mcjj
(författare)
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Balloy, VivianeUniv Calif San Francisco, Dept Med, San Francisco, CA USA.;Inst Pasteur, Unite Def Innee & Inflammat, Paris, France.;INSERM U874, Paris, France.
(författare)
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Fahy, John V.Univ Calif San Francisco, CVRI, San Francisco, CA 94143 USA.
(författare)
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Chignard, MichelInst Pasteur, Unite Def Innee & Inflammat, Paris, France.;INSERM U874, Paris, France.
(författare)
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Imberty, AnneUniv Grenoble, CERMAV CNRS, Grenoble, France.
(författare)
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Wimmerova, MichaelaMasaryk Univ, Cent European Inst Technol, Brno, Czech Republic.;Masaryk Univ, Fac Sci, Natl Ctr Biomol Res, CS-61137 Brno, Czech Republic.;Masaryk Univ, Fac Sci, Dept Biochem, CS-61137 Brno, Czech Republic.
(författare)
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Masaryk Univ, Cent European Inst Technol, Brno, Czech Republic.;Masaryk Univ, Fac Sci, Natl Ctr Biomol Res, CS-61137 Brno, Czech Republic.Masaryk Univ, Fac Sci, Natl Ctr Biomol Res, CS-61137 Brno, Czech Republic.
(creator_code:org_t)
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Ingår i:PLOS ONE: Public Library of Science (PLoS)8:121932-6203
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Houser, Josef
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Komarek, Jan
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Kostlanova, Niko ...
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Cioci, Gianluca
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Varrot, Annabell ...
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Kerr, Sheena C.
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visa fler...
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Lahmann, Martina
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Balloy, Viviane
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Fahy, John V.
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Chignard, Michel
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Imberty, Anne
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Wimmerova, Micha ...
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visa färre...
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