Gold nanoparticles as carriers for a synthetic Streptococcus pneumoniae type 14 conjugate vaccine

Safari, DodiUniv Med Ctr Utrecht, Dept Med Microbiol, Utrecht, Netherlands. (författare)

QC 20211206
Aims: Coupling of capsular polysaccharides of pathogens to immunogenic protein carriers (conjugate vaccines) improves carbohydrate immune response. Our idea is to explore gold nanoclusters as carriers to prepare fully synthetic carbohydrate vaccines. Materials & methods: Gold glyconanoparticles bearing a synthetic tetrasaccharide epitope related to the Streptococcus pneumoniae type 14 capsular polysaccharide (Pn14PS), the T-helper ovalbumin 323-339 peptide (OVA(323-339)), and D-glucose were prepared by a one-pot method. Their immunogenicity was tested in mice. Cytokine levels after spleen cell stimulation with OVA(323-339) were analyzed using a luminex-multiplex cytokine assay. The capacity of the evoked antibodies to promote the uptake of S. pneumoniae type 14 by leukocytes was assessed. Results & discussion: Glyconanoparticles containing 45% of tetrasaccharide and 5% OVA(323-339) triggered specific anti-Pn14PS IgG antibodies. Cytokine levels confirmed that glyconanoparticles led to T-helper cell activation. The antisaccharide antibodies promoted the phagocytosis of type 14 bacteria by human leukocytes, indicating the functionality of the antibodies. Conclusion: Gold nanoparticles have great potential as carriers for the development of a great diversity of fully synthetic carbohydrate-based vaccines.

Marradi, MarcoCIC biomaGUNE, Lab GlycoNanotechnol, Biofunct Nanomat Unit, San Sebastian, Spain.;Networking Res Ctr Bioengn Biomat & Nanomed CIBER, San Sebastian, Spain. (författare)
Chiodo, FabrizioCIC biomaGUNE, Lab GlycoNanotechnol, Biofunct Nanomat Unit, San Sebastian, Spain. (författare)
Dekker, Huberta A. ThUniv Med Ctr Utrecht, Dept Med Microbiol, Utrecht, Netherlands. (författare)
Shan, YulongBangor Univ, Sch Chem, Bangor, Gwynedd, Wales. (författare)
Adamo, RobertoUniv Utrecht, Bijvoet Ctr, Dept Bioorgan Chem, NL-3508 TB Utrecht, Netherlands. (författare)
Oscarson, StefanUniv Coll Dublin, Ctr Synth & Chem Biol, Dublin 4, Ireland. (författare)
Rijkers, Ger T.St Antonius Hosp, Dept Med Microbiol & Immunol, Nieuwegein, Netherlands. (författare)
Lahmann, MartinaBangor Univ, Sch Chem, Bangor, Gwynedd, Wales.(Swepub:kth)u190mcjj (författare)
Kamerling, Johannis P.Univ Utrecht, Bijvoet Ctr, Dept Bioorgan Chem, NL-3508 TB Utrecht, Netherlands. (författare)
Penades, SoledadCIC biomaGUNE, Lab GlycoNanotechnol, Biofunct Nanomat Unit, San Sebastian, Spain.;Networking Res Ctr Bioengn Biomat & Nanomed CIBER, San Sebastian, Spain. (författare)
Snippe, HarmUniv Med Ctr Utrecht, Dept Med Microbiol, Utrecht, Netherlands. (författare)
Univ Med Ctr Utrecht, Dept Med Microbiol, Utrecht, Netherlands.CIC biomaGUNE, Lab GlycoNanotechnol, Biofunct Nanomat Unit, San Sebastian, Spain.;Networking Res Ctr Bioengn Biomat & Nanomed CIBER, San Sebastian, Spain. (creator_code:org_t)

Av författaren/redakt...: Safari, Dodi; Marradi, Marco; Chiodo, Fabrizio; Dekker, Huberta ...; Shan, Yulong; Adamo, Roberto; visa fler...; Oscarson, Stefan; Rijkers, Ger T.; Lahmann, Martina; Kamerling, Johan ...; Penades, Soledad; Snippe, Harm; visa färre...

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