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Spatial Transcripto...
Spatial Transcriptomics to define transcriptional patterns of zonation and structural components in the mouse liver
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- Hildebrandt, Franziska, 1994- (author)
- Stockholms universitet,Institutionen för molekylär biovetenskap, Wenner-Grens institut
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- Andersson, Alma (author)
- KTH,Genteknologi,Science for Life Laboratory, SciLifeLab,KTH Royal Inst Technol, Dept Gene Technol, Sci Life Lab, Tomtebodavagen 23a, SE-17165 Solna, Sweden.
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- Saarenpää, Sami (author)
- KTH,Genteknologi,Science for Life Laboratory, SciLifeLab,KTH Royal Inst Technol, Dept Gene Technol, Sci Life Lab, Tomtebodavagen 23a, SE-17165 Solna, Sweden.
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- Larsson, Ludvig (author)
- KTH,Genteknologi,Science for Life Laboratory, SciLifeLab,KTH Royal Inst Technol, Dept Gene Technol, Sci Life Lab, Tomtebodavagen 23a, SE-17165 Solna, Sweden.
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- Van Hul, Noemi (author)
- Karolinska Institutet,Karolinska Inst Stockholm, Dept Cell & Mol Biol, SE-17177 Solna, Sweden.
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- Kanatani, Sachie, 1982- (author)
- Stockholms universitet,Institutionen för molekylär biovetenskap, Wenner-Grens institut
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- Masek, Jan (author)
- Karolinska Institutet,Karolinska Inst Stockholm, Dept Cell & Mol Biol, SE-17177 Solna, Sweden.;Charles Univ Prague, Dept Cell Biol, Fac Sci, Vinicna 7, Prague 12800 2, Czech Republic.
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- Ellis, Ewa (author)
- Karolinska Institutet,Karolinska Inst, Dept Clin Sci Intervent & Technol CLINTEC, S-14186 Stockholm, Sweden.
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- Barragan, Antonio, 1968- (author)
- Stockholms universitet,Institutionen för molekylär biovetenskap, Wenner-Grens institut
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- Mollbrink, Annelie (author)
- KTH,Science for Life Laboratory, SciLifeLab,Genteknologi,KTH Royal Inst Technol, Dept Gene Technol, Sci Life Lab, Tomtebodavagen 23a, SE-17165 Solna, Sweden.
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- Andersson, Emma R. (author)
- Karolinska Inst Stockholm, Dept Cell & Mol Biol, SE-17177 Solna, Sweden.
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- Lundeberg, Joakim (author)
- KTH,Science for Life Laboratory, SciLifeLab,Genteknologi,KTH Royal Inst Technol, Dept Gene Technol, Sci Life Lab, Tomtebodavagen 23a, SE-17165 Solna, Sweden.
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- Ankarklev, Johan, 1980- (author)
- Stockholms universitet,Uppsala universitet,Institutionen för cell- och molekylärbiologi,Science for Life Laboratory, SciLifeLab,Stockholm Univ, Wenner Gren Inst, Dept Mol Biosci, Svante Arrhenius Vag 20C, SE-10691 Stockholm, Sweden.,Institutionen för molekylär biovetenskap, Wenner-Grens institut
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(creator_code:org_t)
- 2021-12-02
- 2021
- English.
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In: Nature Communications. - : Springer Nature. - 2041-1723. ; 12:1
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Abstract
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- Reconstruction of heterogeneity through single cell transcriptional profiling has greatly advanced our understanding of the spatial liver transcriptome in recent years. However, global transcriptional differences across lobular units remain elusive in physical space. Here, we apply Spatial Transcriptomics to perform transcriptomic analysis across sectioned liver tissue. We confirm that the heterogeneity in this complex tissue is predominantly determined by lobular zonation. By introducing novel computational approaches, we enable transcriptional gradient measurements between tissue structures, including several lobules in a variety of orientations. Further, our data suggests the presence of previously transcriptionally uncharacterized structures within liver tissue, contributing to the overall spatial heterogeneity of the organ. This study demonstrates how comprehensive spatial transcriptomic technologies can be used to delineate extensive spatial gene expression patterns in the liver, indicating its future impact for studies of liver function, development and regeneration as well as its potential in pre-clinical and clinical pathology. Global transcriptional differences across lobular units in the liver remain unknown. Here the authors perform spatial transcriptomics of liver tissue to delineate transcriptional differences in physical space, confirm lobular zonation along transcriptional gradients and suggest the presence of previously uncharacterized structures within liver tissue.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Cancer och onkologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Cancer and Oncology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Genetik (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Genetics (hsv//eng)
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- By the author/editor
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Hildebrandt, Fra ...
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Andersson, Alma
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Saarenpää, Sam ...
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Larsson, Ludvig
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Van Hul, Noemi
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Kanatani, Sachie ...
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show more...
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Masek, Jan
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Ellis, Ewa
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Barragan, Antoni ...
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Mollbrink, Annel ...
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Andersson, Emma ...
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Lundeberg, Joaki ...
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Ankarklev, Johan ...
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- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Clinical Medicin ...
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and Cancer and Oncol ...
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Cell and Molecul ...
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Biochemistry and ...
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Genetics
- Articles in the publication
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Nature Communica ...
- By the university
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Royal Institute of Technology
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Karolinska Institutet
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Uppsala University
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Stockholm University