Bead-Based Assays for Validating Proteomic Profiles in Body Fluids

Bendes, AnnikaKTH,Affinitets-proteomik,Science for Life Laboratory, SciLifeLab (författare)

QC 20220316
Protein biomarkers in biological fluids represent an important resource for improving the clinical management of diseases. Current proteomics technologies are capable of performing high-throughput and multiplex profiling in different types of fluids, often leading to the shortlisting of tens of candidate biomarkers per study. However, before reaching any clinical setting, these discoveries require thorough validation and an assay that would be suitable for routine analyses. In the path from biomarker discovery to validation, the performance of the assay implemented for the intended protein quantification is extremely critical toward achieving reliable and reproducible results. Development of robust sandwich immunoassays for individual candidates is challenging and labor and resource intensive, and multiplies when evaluating a panel of interesting candidates at the same time. Here we describe a versatile pipeline that facilitates the systematic and parallel development of multiple sandwich immunoassays using a bead-based technology.

NATURVETENSKAP Biologi Biokemi och molekylärbiologi hsv//swe
NATURAL SCIENCES Biological Sciences Biochemistry and Molecular Biology hsv//eng
Antibody
Mass spectrometry (MS)
Protein standard
Sandwich immunoassay (SIA)
Single-binder immunoassays
Suspension bead array (SBA)
bead based assay
body fluid
immunoassay
mass spectrometry
protein determination
protein fingerprinting
proteomics
chemistry
enzyme linked immunosorbent assay
human
biological marker
proteome
Biomarkers
Body Fluids
Enzyme-Linked Immunosorbent Assay
Humans

Dale, MatildaKTH,Affinitets-proteomik,Science for Life Laboratory, SciLifeLab(Swepub:kth)u1n0viuj (författare)
Mattsson, CeciliaKTH,Affinitets-proteomik(Swepub:kth)u1adnwkp (författare)
Dodig-Crnkovic, TeaKTH,Affinitets-proteomik,Science for Life Laboratory, SciLifeLab(Swepub:kth)u1wrth1x (författare)
Iglesias, Maria JesusKTH,Science for Life Laboratory, SciLifeLab,Cellulär och klinisk proteomik,Department of Clinical Medicine, Faculty of Health ScienceThe Arctic University of Tromsö, Tromsö Norway(Swepub:kth)u1b212k2 (författare)
Schwenk, Jochen M.KTH,Science for Life Laboratory, SciLifeLab,Affinitets-proteomik(Swepub:kth)u1h7wtme (författare)
Fredolini, ClaudiaKTH,Science for Life Laboratory, SciLifeLab,Affinitets-proteomik(Swepub:kth)u11xul3s (författare)
KTHAffinitets-proteomik (creator_code:org_t)

Ingår i:Protein Microarrays for Disease AnalysisNew York, NY : Springer Nature, s. 65-78

Av författaren/redakt...: Bendes, Annika; Dale, Matilda; Mattsson, Cecili ...; Dodig-Crnkovic, ...; Iglesias, Maria ...; Schwenk, Jochen ...; visa fler...; Fredolini, Claud ...; visa färre...

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