Bead-Based Assays for Validating Proteomic Profiles in Body Fluids

• Protein biomarkers in biological fluids represent an important resource for improving the clinical management of diseases. Current proteomics technologies are capable of performing high-throughput and multiplex profiling in different types of fluids, often leading to the shortlisting of tens of candidate biomarkers per study. However, before reaching any clinical setting, these discoveries require thorough validation and an assay that would be suitable for routine analyses. In the path from biomarker discovery to validation, the performance of the assay implemented for the intended protein quantification is extremely critical toward achieving reliable and reproducible results. Development of robust sandwich immunoassays for individual candidates is challenging and labor and resource intensive, and multiplies when evaluating a panel of interesting candidates at the same time. Here we describe a versatile pipeline that facilitates the systematic and parallel development of multiple sandwich immunoassays using a bead-based technology. 

Subject headings

NATURVETENSKAP  -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)

NATURAL SCIENCES  -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)

Keyword

Antibody

Mass spectrometry (MS)

Protein standard

Sandwich immunoassay (SIA)

Single-binder immunoassays

Suspension bead array (SBA)

bead based assay

body fluid

immunoassay

mass spectrometry

protein determination

protein fingerprinting

proteomics

chemistry

enzyme linked immunosorbent assay

human

biological marker

proteome

Biomarkers

Body Fluids

Enzyme-Linked Immunosorbent Assay

Humans

Publication and Content Type

ref (subject category)

kap (subject category)