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Sökning: WFRF:(Jakobsson S) > Medicin och hälsovetenskap > Notarnicola Antonella > Lundstrom Susanna L. > Longitudinal assess...

  • Notarnicola, AntonellaKarolinska Institutet (författare)

Longitudinal assessment of reactivity and affinity profile of anti-Jo1 autoantibodies to distinct HisRS domains and a splice variant in a cohort of patients with myositis and anti-synthetase syndrome

  • Artikel/kapitelEngelska2022

Förlag, utgivningsår, omfång ...

  • 2022-03-02
  • Springer Nature,2022
  • printrdacarrier

Nummerbeteckningar

  • LIBRIS-ID:oai:DiVA.org:kth-310073
  • https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-310073URI
  • https://doi.org/10.1186/s13075-022-02745-6DOI
  • http://kipublications.ki.se/Default.aspx?queryparsed=id:148962436URI

Kompletterande språkuppgifter

  • Språk:engelska
  • Sammanfattning på:engelska

Ingår i deldatabas

Klassifikation

  • Ämneskategori:ref swepub-contenttype
  • Ämneskategori:art swepub-publicationtype

Anmärkningar

  • QC 20220321
  • Background To address the reactivity and affinity against histidyl-transfer RNA synthetase (HisRS) autoantigen of anti-Jo1 autoantibodies from serum and bronchoalveolar lavage fluid (BALF) in patients with idiopathic inflammatory myopathies/anti-synthetase syndrome (IIM/ASSD). To investigate the associations between the reactivity profile and clinical data over time. Methods Samples and clinical data were obtained from (i) 25 anti-Jo1(+) patients (19 sera with 16 longitudinal samples and 6 BALF/matching sera at diagnosis), (ii) 29 anti-Jo1(-) patients (25 sera and 4 BALF/matching sera at diagnosis), and (iii) 27 age/gender-matched healthy controls (24 sera and 3 BALF/matching sera). Reactivity towards HisRS full-length (HisRS-FL), three HisRS domains (WHEP, antigen binding domain (ABD), and catalytic domain (CD)), and the HisRS splice variant (SV) was tested. Anti-Jo1 IgG reactivity was evaluated by ELISA and western blot using IgG purified from serum by affinity chromatography. In paired serum-BALF, anti-Jo1 IgG and IgA reactivity was analyzed by ELISA. Autoantibody affinity was measured by surface plasmon resonance using IgG purified from sera. Correlations between autoantibody reactivity and clinical data were evaluated at diagnosis and longitudinally. Results Anti-Jo1 IgG from serum and BALF bound HisRS-FL, WHEP, and SV with high reactivity at the time of diagnosis and recognized both conformation-dependent and conformation-independent HisRS epitopes. Anti-HisRS-FL IgG displayed high affinity early in the disease. At the time of IIM/ASSD diagnosis, the highest autoantibody levels against HisRS-FL were found in patients ever developing interstitial lung disease (ILD) and arthritis, but with less skin involvement. Moreover, the reactivity of anti-WHEP IgG in BALF correlated with poor pulmonary function. Levels of autoantibodies against HisRS-FL, HisRS domains, and HisRS splice variant generally decreased over time. With some exceptions, longitudinal anti-HisRS-FL antibody levels changed in line with ILD activity. Conclusion High levels and high-affinity anti-Jo1 autoantibodies towards HisRS-FL were found early in disease in sera and BALF. In combination with the correlation of anti-HisRS-FL antibody levels with ILD and ILD activity in longitudinal samples as well as of anti-WHEP IgG in BALF with poor pulmonary function, this supports the previously raised hypothesis that the lung might have a role in the immune reaction in anti-Jo1-positive patients.

Ämnesord och genrebeteckningar

Biuppslag (personer, institutioner, konferenser, titlar ...)

  • Preger, CharlottaKarolinska Institutet (författare)
  • Lundstrom, Susanna L.Karolinska Institutet (författare)
  • Renard, NuriaKarolinska Inst, Karolinska Univ Hosp, Dept Med, Div Rheumatol, SE-17164 Stockholm, Sweden.;Karolinska Inst, Ctr Mol Med, Stockholm, Sweden. (författare)
  • Wigren, EdvardKarolinska Inst, Karolinska Univ Hosp, Dept Med, Div Rheumatol, SE-17164 Stockholm, Sweden.;Karolinska Inst, Ctr Mol Med, Stockholm, Sweden.;Struct Genom Consortium, Toronto, ON, Canada. (författare)
  • Van Gompel, EvelineKarolinska Inst, Karolinska Univ Hosp, Dept Med, Div Rheumatol, SE-17164 Stockholm, Sweden.;Karolinska Inst, Ctr Mol Med, Stockholm, Sweden.;KULeuven, Lab Tissue Homeostasis & Dis, Skeletal Biol & Engn Res Ctr, Leuven, Belgium. (författare)
  • Galindo-Feria, Angeles S.Karolinska Institutet (författare)
  • Persson, HelenaKTH,Läkemedelsutveckling,Science for Life Laboratory, SciLifeLab(Swepub:kth)u1l700sh (författare)
  • Fathi, MaryamKarolinska Inst, Karolinska Univ Hosp, Dept Resp Med & Allergy, Bioclinicum, J7 30, SE-17176 Stockholm, Sweden. (författare)
  • Grunewald, JohanKarolinska Institutet (författare)
  • Jakobsson, Per-JohanKarolinska Institutet (författare)
  • Gräslund, SusanneKarolinska Inst, Karolinska Univ Hosp, Dept Med, Div Rheumatol, SE-17164 Stockholm, Sweden.;Karolinska Inst, Ctr Mol Med, Stockholm, Sweden.;Struct Genom Consortium, Toronto, ON, Canada. (författare)
  • Lundberg, Ingrid E.Karolinska Institutet (författare)
  • Fernandes-Cerqueira, CatiaKarolinska Inst, Karolinska Univ Hosp, Dept Med, Div Rheumatol, SE-17164 Stockholm, Sweden.;Karolinska Inst, Ctr Mol Med, Stockholm, Sweden.;4Dcell, 14 Rue La Beaune, F-93100 Montreuil, France. (författare)
  • Karolinska InstitutetKarolinska Inst, Karolinska Univ Hosp, Dept Med, Div Rheumatol, SE-17164 Stockholm, Sweden.;Karolinska Inst, Ctr Mol Med, Stockholm, Sweden. (creator_code:org_t)

Sammanhörande titlar

  • Ingår i:Arthritis Research & Therapy: Springer Nature24:11478-63541478-6362

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