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Sample-to-answer CO...
Sample-to-answer COVID-19 nucleic acid testing using a low-cost centrifugal microfluidic platform with bead-based signal enhancement and smartphone read-out
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- Soares, Ruben R. G. (författare)
- KTH,Science for Life Laboratory, SciLifeLab,Nanobioteknologi
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- Akhtar, Ahmad Saleem (författare)
- KTH,Nanobioteknologi,Science for Life Laboratory, SciLifeLab
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- Pinto, Ines Fernandes (författare)
- KTH,Nanobioteknologi,Science for Life Laboratory, SciLifeLab
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- Lapins, Noa (författare)
- KTH,Nanobioteknologi,Science for Life Laboratory, SciLifeLab
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- Barrett, Donal (författare)
- Karolinska Institutet
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- Sandh, Gustaf (författare)
- Department of Clinical Microbiology, Karolinska University Hospital, Stockholm, Sweden
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- Yin, Xiushan (författare)
- Science for Life Laboratory, Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Solna, Sweden; Applied Biology Laboratory, Shenyang University of Chemical Technology, Shenyang, China; Biotech and Biomedicine Science Co. Ltd, Shenyang, China.
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- Pelechano, Vicent (författare)
- Karolinska Institutet
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- Russom, Aman, Prof. 1976- (författare)
- KTH,Science for Life Laboratory, SciLifeLab,Nanobioteknologi,AIMES – Center for the Advancement of Integrated Medical and Engineering Sciences at Karolinska Institutet and, KTH Royal Institute of Technology, Stockholm, Sweden.
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(creator_code:org_t)
- 2021
- 2021
- Engelska.
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Ingår i: Lab on a Chip. - : Royal Society of Chemistry (RSC). - 1473-0197 .- 1473-0189. ; 21:15, s. 2932-2944
- Relaterad länk:
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https://doi.org/10.1...
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https://pubs.rsc.org...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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http://kipublication...
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Abstract
Ämnesord
Stäng
- With its origin estimated around December 2019 in Wuhan, China, the ongoing SARS-CoV-2 pandemic is a major global health challenge. The demand for scalable, rapid and sensitive viral diagnostics is thus particularly pressing at present to help contain the rapid spread of infection and prevent overwhelming the capacity of health systems. While high-income countries have managed to rapidly expand diagnostic capacities, such is not the case in resource-limited settings of low- to medium-income countries. Aiming at developing cost-effective viral load detection systems for point-of-care COVID-19 diagnostics in resource-limited and resource-rich settings alike, we report the development of an integrated modular centrifugal microfluidic platform to perform loop-mediated isothermal amplification (LAMP) of viral RNA directly from heat-inactivated nasopharyngeal swab samples. The discs were pre-packed with driedn-benzyl-n-methylethanolamine modified agarose beads used to selectively remove primer dimers, inactivate the reaction post-amplification and allowing enhanced fluorescence detectionviaa smartphone camera. Sample-to-answer analysis within 1 hour from sample collection and a detection limit of approximately 100 RNA copies in 10 μL reaction volume were achieved. The platform was validated with a panel of 162 nasopharyngeal swab samples collected from patients with COVID-19 symptoms, providing a sensitivity of 96.6% (82.2-99.9%, 95% CI) for samples with Ct values below 26 and a specificity of 100% (90-100%, 95% CI), thus being fit-for-purpose to diagnose patients with a high risk of viral transmission. These results show significant promise towards bringing routine point-of-care COVID-19 diagnostics to resource-limited settings.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Infektionsmedicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Infectious Medicine (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Medicinsk bioteknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Medical Biotechnology (hsv//eng)
Nyckelord
- Alkanolamines
- Centrifugation
- Cost effectiveness
- Costs
- Diagnosis
- Diseases
- RNA
- Smartphones
- Centrifugal microfluidic platform
- Detection limits
- Enhanced fluorescence
- Loop mediated isothermal amplifications
- Reaction volume
- Sample collection
- Signal enhancement
- Smart-phone cameras
- Microfluidics
- virus RNA
- genetics
- human
- molecular diagnosis
- nucleic acid amplification
- sensitivity and specificity
- smartphone
- COVID-19
- COVID-19 Testing
- Humans
- Molecular Diagnostic Techniques
- Nucleic Acid Amplification Techniques
- RNA
- Viral
- SARS-CoV-2
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Soares, Ruben R. ...
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Akhtar, Ahmad Sa ...
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Pinto, Ines Fern ...
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Lapins, Noa
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Barrett, Donal
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Sandh, Gustaf
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Yin, Xiushan
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Pelechano, Vicen ...
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Russom, Aman, Pr ...
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- Om ämnet
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- NATURVETENSKAP
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NATURVETENSKAP
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och Biologi
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och Biokemi och mole ...
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Klinisk medicin
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och Infektionsmedici ...
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Medicinsk biotek ...
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och Medicinsk biotek ...
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Lab on a Chip
- Av lärosätet
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Kungliga Tekniska Högskolan
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Karolinska Institutet