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Putting Humpty Dump...
Putting Humpty Dumpty Back Together Again : What Does Protein Quantification Mean in Bottom-Up Proteomics? br
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- Plubell, Deanna L. (author)
- Univ Washington, Dept Genome Sci, Seattle, WA 98195 USA.
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- Käll, Lukas, 1969- (author)
- KTH,Science for Life Laboratory, SciLifeLab,Genteknologi
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- Webb-Robertson, Bobbie-Jo (author)
- Pacific Northwest Natl Lab, Richland, WA 99352 USA.
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- Bramer, Lisa M. (author)
- Pacific Northwest Natl Lab, Richland, WA 99352 USA.
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- Ives, Ashley (author)
- Northwestern Univ, Prote Ctr Excellence, Evanston, IL 60208 USA.;Northwestern Univ, Dept Chem, Evanston, IL 60208 USA.;Northwestern Univ, Dept Mol Biosci, Evanston, IL 60208 USA.
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- Kelleher, Neil L. (author)
- Northwestern Univ, Prote Ctr Excellence, Evanston, IL 60208 USA.;Northwestern Univ, Dept Chem, Evanston, IL 60208 USA.;Northwestern Univ, Dept Mol Biosci, Evanston, IL 60208 USA.
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- Smith, Lloyd M. (author)
- Univ Wisconsin, Dept Chem, Madison, WI 53706 USA.
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- Montine, Thomas J. (author)
- Stanford Univ, Dept Pathol, Stanford, CA 94305 USA.
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- Wu, Christine C. (author)
- Univ Washington, Dept Genome Sci, Seattle, WA 98195 USA.
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- MacCoss, Michael J. (author)
- Univ Washington, Dept Genome Sci, Seattle, WA 98195 USA.
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Univ Washington, Dept Genome Sci, Seattle, WA 98195 USA Science for Life Laboratory, SciLifeLab (creator_code:org_t)
- 2022-02-27
- 2022
- English.
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In: Journal of Proteome Research. - : American Chemical Society (ACS). - 1535-3893 .- 1535-3907. ; 21:4, s. 891-898
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https://doi.org/10.1...
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Abstract
Subject headings
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- Bottom-up proteomics provides peptide measurements and has beeninvaluable for moving proteomics into large-scale analyses. Commonly, a singlequantitative value is reported for each protein-coding gene by aggregating peptidequantities into protein groups following protein inference or parsimony. However, giventhe complexity of both RNA splicing and post-translational protein modification, it isoverly simplistic to assume that all peptides that map to a singular protein-coding genewill demonstrate the same quantitative response. By assuming that all peptides from aprotein-coding sequence are representative of the same protein, we may miss thediscovery of important biological differences. To capture the contributions of existingproteoforms, we need to reconsider the practice of aggregating protein values to a singlequantity per protein-coding gene.
Subject headings
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Keyword
- quantitative proteomics
- proteoforms
- post-translational modifications
- quantitative analysis
- protein grouping
Publication and Content Type
- ref (subject category)
- art (subject category)
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To the university's database
- By the author/editor
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Plubell, Deanna ...
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Käll, Lukas, 196 ...
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Webb-Robertson, ...
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Bramer, Lisa M.
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Ives, Ashley
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Kelleher, Neil L ...
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show more...
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Smith, Lloyd M.
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Montine, Thomas ...
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Wu, Christine C.
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MacCoss, Michael ...
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- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Biochemistry and ...
- Articles in the publication
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Journal of Prote ...
- By the university
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Royal Institute of Technology