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Clustering and cros...
Clustering and cross-linking of the wheat storage protein α-gliadin : A combined experimental and theoretical approach
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- Markgren, Joel (author)
- Sveriges lantbruksuniversitet,Swedish University of Agricultural Sciences,Institutionen för växtförädling,Department of Plant Breeding
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- Rasheed, Faiza (author)
- Sveriges lantbruksuniversitet,Swedish University of Agricultural Sciences,KTH Royal Institute of Technology,KTH,Polymera material,Swedish Univ Agr Sci, Dept Plant Breeding, POB 190, SE-23422 Lomma, Sweden.;Quaid I Azam Univ, Dept Biotechnol, Islamabad, Pakistan.,Institutionen för växtförädling,Department of Plant Breeding,Royal Institute of Technology
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- Hedenqvist, Mikael S. (author)
- KTH Royal Institute of Technology,KTH,Polymera material
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- Skepö, Marie (author)
- Lund University,Lunds universitet,Beräkningskemi,Enheten för fysikalisk och teoretisk kemi,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,LINXS - Institute of advanced Neutron and X-ray Science,LTH profilområde: Nanovetenskap och halvledarteknologi,LTH profilområden,Computational Chemistry,Physical and theoretical chemistry,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH,Faculty of Engineering, LTH,LTH Profile Area: Nanoscience and Semiconductor Technology,LTH Profile areas,Faculty of Engineering, LTH
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- Johansson, Eva (author)
- Sveriges lantbruksuniversitet,Swedish University of Agricultural Sciences,Institutionen för växtförädling,Department of Plant Breeding
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(creator_code:org_t)
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- Elsevier BV, 2022
- 2022
- English.
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In: International Journal of Biological Macromolecules. - : Elsevier BV. - 0141-8130 .- 1879-0003. ; 211, s. 592-615
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Abstract
Subject headings
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- Our aim was to understand mechanisms for clustering and cross-linking of gliadins, a wheat seed storage protein type, monomeric in native state, but incorporated in network while processed. The mechanisms were studied utilizing spectroscopy and high-performance liquid chromatography on a gliadin-rich fraction, in vitro produced alpha-gliadins, and synthetic gliadin peptides, and by coarse-grained modelling, Monte Carlo simulations and prediction algorithms. In solution, gliadins with alpha-helix structures (dip at 205 nm in CD) were primarily present as monomeric molecules and clusters of gliadins (peaks at 650- and 700-s on SE-HPLC). At drying, large polymers (Rg 90.3 nm by DLS) were formed and 13-sheets increased (14% by FTIR). Trained algorithms predicted aggregation areas at amino acids 115-140, 150-179, and 250-268, and induction of liquid-liquid phase separation at P- and Poly-Q-sequences (Score = 1). Simulations showed that gliadins formed polymers by tail-to-tail or a hydrophobic core (Kratky plots and Ree = 35 and 60 for C- and N-terminal). Thus, the N-terminal formed clusters while the C-terminal formed aggregates by disulphide and lanthionine bonds, with favoured hydrophobic clustering of similar/exact peptide sections (synthetic peptide mixtures on SE-HPLC). Mechanisms of clustering and cross-linking of the gliadins presented here, contribute ability to tailor processing results, using these proteins.
Subject headings
- NATURVETENSKAP -- Kemi -- Polymerkemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Polymer Chemistry (hsv//eng)
- NATURVETENSKAP -- Kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Keyword
- Disulphide bonds
- Monte Carlo simulations
- Polymers
- Synthetic peptides
- Disulphide bonds
- Monte Carlo simulations
- Polymers
- Synthetic peptides
Publication and Content Type
- ref (subject category)
- art (subject category)
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