3D Bioprinting of Multi-Material Decellularized Liver Matrix Hydrogel at Physiological Temperatures

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Khati, VamakshiKTH,Nanobioteknologi,Science for Life Laboratory, SciLifeLab (författare)

3D Bioprinting of Multi-Material Decellularized Liver Matrix Hydrogel at Physiological Temperatures

Artikel/kapitelEngelska2022

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2022-07-13
MDPI AG,2022
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LIBRIS-ID:oai:DiVA.org:kth-316289
https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-316289URI
https://doi.org/10.3390/bios12070521DOI
http://kipublications.ki.se/Default.aspx?queryparsed=id:150307227URI

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Språk:engelska
Sammanfattning på:engelska

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Anmärkningar

QC 20220812
Bioprinting is an acclaimed technique that allows the scaling of 3D architectures in an organized pattern but suffers from a scarcity of appropriate bioinks. Decellularized extracellular matrix (dECM) from xenogeneic species has garnered support as a biomaterial to promote tissue-specific regeneration and repair. The prospect of developing dECM-based 3D artificial tissue is impeded by its inherent low mechanical properties. In recent years, 3D bioprinting of dECM-based bioinks modified with additional scaffolds has advanced the development of load-bearing constructs. However, previous attempts using dECM were limited to low-temperature bioprinting, which is not favorable for a longer print duration with cells. Here, we report the development of a multi-material decellularized liver matrix (dLM) bioink reinforced with gelatin and polyethylene glycol to improve rheology, extrudability, and mechanical stability. This shear-thinning bioink facilitated extrusion-based bioprinting at 37 degrees C with HepG2 cells into a 3D grid structure with a further enhancement for long-term applications by enzymatic crosslinking with mushroom tyrosinase. The heavily crosslinked structure showed a 16-fold increase in viscosity (2.73 Pa s(-1)) and a 32-fold increase in storage modulus from the non-crosslinked dLM while retaining high cell viability (85-93%) and liver-specific functions. Our results show that the cytocompatible crosslinking of dLM bioink at physiological temperatures has promising applications for extended 3D-printing procedures.

Ämnesord och genrebeteckningar

NATURVETENSKAP Biologi hsv//swe
NATURAL SCIENCES Biological Sciences hsv//eng
decellularized liver matrix bioink
bioprinting at physiological temperatures
cytocompatible crosslinking
robust bioink
viscoelasticity

Biuppslag (personer, institutioner, konferenser, titlar ...)

Ramachandraiah, HarishaBiopr AB, S-17165 Solna, Sweden. (författare)
Pati, FalguniIndian Inst Technol Hyderabad, Dept Biomed Engn, Kandi 502285, India. (författare)
Svahn Andersson, HeleneKTH,Nanobioteknologi,Science for Life Laboratory, SciLifeLab(Swepub:kth)u1706qv6 (författare)
Gaudenzi, GiuliaKarolinska Institutet,KTH,Nanobioteknologi,Science for Life Laboratory, SciLifeLab,Department of Global Public Health, Karolinska Institute, 17165 Solna, Sweden(Swepub:kth)u13tsjp0 (författare)
Russom, Aman,Prof.1976-Karolinska Institutet,KTH,Nanobioteknologi,Science for Life Laboratory, SciLifeLab,AIMES—Center for the Advancement of Integrated Medical and Engineering Sciences, Karolinska Institute and KTH Royal Institute of Technology, 11428 Stockholm, Sweden(Swepub:kth)u1go6jxm (författare)
KTHNanobioteknologi (creator_code:org_t)

Sammanhörande titlar

Ingår i:Biosensors: MDPI AG12:72079-6374

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Av författaren/redakt...: Khati, Vamakshi; Ramachandraiah, ...; Pati, Falguni; Svahn Andersson, ...; Gaudenzi, Giulia; Russom, Aman, Pr ...

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Artiklar i publikationen: Biosensors

Av lärosätet: Kungliga Tekniska Högskolan; Karolinska Institutet

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