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Targeted Proteomics...
Targeted Proteomics Using Stable Isotope Labeled Protein Fragments Enables Precise and Robust Determination of Total Apolipoprotein(a) in Human Plasma
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- Hober, Andreas, 1992- (författare)
- KTH,Proteinvetenskap,Science for Life Laboratory, SciLifeLab
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- Rekanovic, Mirela (författare)
- AstraZeneca, Gothenburg, Sweden,Translational Science and Experimental Medicine, Cardiovascular, Renal and Metabolism, IMED Biotech Unit
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- Forsström, Björn (författare)
- KTH,Science for Life Laboratory, SciLifeLab,Proteinvetenskap
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- Hansson, Sara (författare)
- AstraZeneca, Gothenburg, Sweden,Translational Science and Experimental Medicine, Cardiovascular, Renal and Metabolism, IMED Biotech Unit
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- Kotol, David (författare)
- KTH,Science for Life Laboratory, SciLifeLab,Proteinvetenskap
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- Percy, Andrew J (författare)
- Cambridge Isotope Laboratories, Inc.,Department of Applications Development
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- Uhlén, Mathias (författare)
- KTH,Science for Life Laboratory, SciLifeLab,Proteinvetenskap
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- Oscarsson, Jan (författare)
- AstraZeneca, Gothenburg, Sweden,Late-stage Development, Cardiovascular, Renal and Metabolism, Biopharmaceuticals R&D
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- Edfors, Fredrik (författare)
- KTH,Proteinvetenskap,Science for Life Laboratory, SciLifeLab
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- Miliotis, Tasso (författare)
- AstraZeneca, Gothenburg, Sweden,Translational Science and Experimental Medicine, Cardiovascular, Renal and Metabolism, IMED Biotech Unit
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(creator_code:org_t)
- Engelska.
- Relaterad länk:
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https://kth.diva-por...
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https://urn.kb.se/re...
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Abstract
Ämnesord
Stäng
- Lipoprotein(a), also known as Lp(a), is an LDL-like particle composed of apolipoprotein(a) (apo(a)) bound covalently to apolipoprotein B100. Plasma concentrations of Lp(a) are highly heritable and vary widely between individuals. Elevated plasma concentration of Lp(a) is considered as an independent, causal risk factor of cardiovascular disease (CVD). Targeted mass spectrometry (LC-SRM/MS) combined with stable isotope-labeled recombinant proteins provides robust and precise quantification of proteins in the blood, making LC-SRM/MS assays appealing for monitoring plasma proteins for clinical implications. This study presents a novel quantitative approach, based on proteotypic peptides, to determine the absolute concentration of apo(a) from two microliters of plasma and qualified according to guideline requirements for targeted proteomics assays. After optimization, assay parameters such as linearity, lower limits of quantification (LLOQ), intra-assay variability (CV: 4.7%) and inter-assay repeatability (CV: 7.8%) were determined and the LC-SRM/MS results were benchmarked against a commercially available immunoassay. In summary, the measurements of an apo(a) single copy specific peptide and a kringle 4 specific peptide allows for the determination of molar concentration and relative size of apo(a) in individuals.
Ämnesord
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
Nyckelord
- apolipoprotein(a) (apo(a))
- apolipoproteins
- quantification
- stable isotope labeled recombinant protein fragment standard
- liquid chromatography
- tandem mass spectrometry
- Biotechnology
- Bioteknologi
Publikations- och innehållstyp
- vet (ämneskategori)
- ovr (ämneskategori)
- Av författaren/redakt...
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Hober, Andreas, ...
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Rekanovic, Mirel ...
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Forsström, Björn
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Hansson, Sara
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Kotol, David
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Percy, Andrew J
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visa fler...
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Uhlén, Mathias
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Oscarsson, Jan
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Edfors, Fredrik
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Miliotis, Tasso
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visa färre...
- Om ämnet
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- NATURVETENSKAP
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NATURVETENSKAP
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och Biologi
- Av lärosätet
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Kungliga Tekniska Högskolan